2016 Fiscal Year Annual Research Report
１神経細胞における機能的ジーンサイレンシングの可視化
| Project/Area Number |
15F15711
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| Research Institution | Kyoto University |
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Principal Investigator |
影山 龍一郎 京都大学, ウイルス・再生医科学研究所, 教授 (80224369)
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| Co-Investigator(Kenkyū-buntansha) |
GOLDIE BELINDA 京都大学, ウイルス・再生医科学研究所, 外国人特別研究員
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| Project Period (FY) |
2015-07-29 – 2017-03-31
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| Keywords | Neurobiology / RNA dynamics / synaptic plasticity / learning and memory |
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| Outline of Annual Research Achievements |
The difficulties with defining a successful imaging protocol for the cell line used in this project have eventually been overcome and, although this took a little longer than expected, we have been able to proceed quite rapidly with the imaging experiments. The fellow has a manuscript in progress that will be able to completed after her return to Australia. As there is only have 1 month remaining on this fellowship, the fellow is reaching the end of experimental work here in Japan. The current status of her research is as follows.
1) Submission of manuscript for a project begun in Australia and completed in Japan with new technique established in the host laboratory during year 1 of the fellowship. 2) Refinement of a protocol for confocal imaging of the human neuronal cell line being used for the current project. 3) Learned new Reprogramming techniques for analysing m6A RNA modification by next-generation sequencing data. 4) Learned protein analysis techniques that have revealed unexpected and exciting information about the translation control pathways used in response to neuronal activity. 5) Co-author on a manuscript about to be submitted by the host lab. 6) Manuscript in progress from own project.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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