2017 Fiscal Year Annual Research Report
| Project/Area Number |
15F15780
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Carninci Piero 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長 (10333296)
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| Co-Investigator(Kenkyū-buntansha) |
BUDIC MARUSKA 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
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| Project Period (FY) |
2015-11-09 – 2018-03-31
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| Keywords | SINEUP / upregulation / transcription factor / iPS / reprogramming / lentivirus |
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| Outline of Annual Research Achievements |
I designed and optimized the synthetic SINEUPs for reprogramming human iPS cells (hiPSCs) into neuron cells and tested their activity in HEK293T/17 cells. Since lipid-mediated plasmid delivery has low efficiency in hiPSCs compared to viral delivery methods, I constructed four lentiviral vectors to express transcription factor specific (NGN2, NGN3, TLX3, PAX6) SINEUPs to enhance translation of endogenous TF mRNA in hiPSCs in order to reprogram them into neurons. I transduced hiPSCs with one SINEUP or a coctail of two, three, or four diferent SINEUPs. There were clear differences in terms of neuron differentiation. Coctails of at least two different SINEUPs have a potential for hiPSC reprogramming into neurons.
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| Research Progress Status |
29年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
29年度が最終年度であるため、記入しない。
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