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2007 Fiscal Year Annual Research Report

時空間における分子振動計測の極限化:分子から細胞まで物質組織化機構の解明に向けて

Research Project

Project/Area Number 15GS0204
Research InstitutionThe University of Tokyo

Principal Investigator

濱口 宏夫  The University of Tokyo, 大学院・理学系研究科, 教授 (00092297)

Co-Investigator(Kenkyū-buntansha) 岩澤 康裕  東京大学, 大学院・理学系研究科, 教授 (40018015)
岩田 耕一  東京大学, 大学院・理学系研究科, 准教授 (90232678)
加納 英明  東京大学, 大学院・理学系研究科, 准教授 (70334240)
田原 太平  東京大学, 理化学研究所分子分光研究室, 主任研究員 (60217164)
高塚 和夫  東京大学, 大学院・総合文化研究科, 教授 (70154797)
Keywords時間分解分光 / 空間分解分光 / 時空間分解分光 / ラマン分光 / 非線形ラマン分光 / 顕微分光 / ハイパーラマン散乱 / 分子近接場効果
Research Abstract

平成19年度の研究の過程で、β-カロテンなど対称心を持つ共役色素の電子遷移が、近接する溶媒分子のハイパーラマン散乱強度を顕著に増大させるという新しい現象(分子近接場効果)を発見した。分子近接場効果を用いると、プローブ分子の近傍のみを選択的に計測する新しいナノメートル局所振動分光法が誕生する可能性がある。この可能性をさらに探求するために、研究計画を当初計画(平成19年度終了)より1年間(平成20年度まで)延長することを申請し、承認された。延長後の1年間に、ハイパーラマン散乱測定装置の高速化および励起波長の可変化に取り組み、β-カロテンの共鳴ハイパーラマン散乱の励起プロファイルを観測して、先に提出していた分子近接場効果の理論を裏付ける結果を得た。また、分子近接場効果を示す溶媒のスペクトルが、バルクのものと顕著に異なることを見出した。さらに、より簡単な色素であるヨウ素も分子近接場効果を示すことを見出し、この効果が一般性を持つことを確かめた。このように、1年間の研究期間延長により、分子近接場効果の機構に対する理解が深まり、ナノメートル、フェムト秒の時空間分解振動分光の実現に向けて一歩前進することができた。平成19および20年度を通じて、ピコ秒時間分解ラマン分光による光励起されたtrans-スチルベン分子の振動冷却過程の研究をさらに進展させた。6種のイオン液体中での冷却曲線を得て、これを熱拡散方程式により解析し、イオン液体中の局所構造のサイズに関する情報を得た。顕微CARS分光システムの開発をさらに推進し、近赤外のサブピコ秒レーザーを用いた装置を新たに開発し、従来測定が困難であった生細胞の指紋領域での分光イメージングを可能にした。近赤外共焦点顕微ラマン分光装置を製作し、植物細胞など蛍光の妨害により顕微ラマン分光測定が不可能な生体試料の振動スペクトル測定を可能にした。

  • Research Products

    (31 results)

All 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (17 results)

  • [Journal Article] High concentration trans form unsaturated lipids detected in a HeLa cell by Raman microspectroscopy2008

    • Author(s)
      Chikao Onogi, Michiyo Motoyama, Hiro-o Hamaguchi
    • Journal Title

      Journal of Raman Spectroscopy 39・5

      Pages: 555-556

    • Peer Reviewed
  • [Journal Article] Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared2008

    • Author(s)
      Masanari Okuno, Hideaki Kano, Pilippe Leproux, Vincent Courdec, Hiro-o Hamaguchi
    • Journal Title

      Optics Letters 33・9

      Pages: 923-925

    • Peer Reviewed
  • [Journal Article] Intensity enhancement and selectivedetection of proximate solvent molecules by molecular near-field effect in resonance hyper-Raman scattering2008

    • Author(s)
      Rintaro Shimada, Hideaki Kano, Hiro-o Hamaguchi
    • Journal Title

      Journal of Chemical Physics 129・2

      Pages: 24505

    • Peer Reviewed
  • [Journal Article] Molecular vibrational imaging of a human cell by multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum light source2008

    • Author(s)
      Hideaki Kano
    • Journal Title

      Journal of Raman Spectroscopy 39・11

      Pages: 1649-1652

    • Peer Reviewed
  • [Journal Article] Structural change of 1-butyl-3-methylimidazolium tetrafluoroborate plus water mixtures studied by infrared vibrational Spectroscopy2008

    • Author(s)
      Yoonnam Jeon, Jaeho Sung, Doseok Kim, Chungwon Seo, Hyeonsik Cheong, Yukio Ouchi, Ryosuke Ozawa, Hiro-o Hamaguchi
    • Journal Title

      Jounal of Physical Chemistry B 112・3

      Pages: 923-928

    • Peer Reviewed
  • [Journal Article] In vivo measurement of human dermis by 1064nm-excited fiber Raman spectroscopy2008

    • Author(s)
      Satoshi Naito, Youn-Kun Min, K Sugata, O Osanai, T Kitahara, Hideo Hiruma, Hiro-o Hamaguchi
    • Journal Title

      Skin Research and Technology 14・1

      Pages: 18-25

    • Peer Reviewed
  • [Journal Article] Development of an automatic phase-contrast microscopic system capable of determining the microbial density and distribution inside an immobilized carrier2008

    • Author(s)
      Yong-Woo Lee, Jong-Kwang Lee, Youn-Kun Min, Hiro-o Hamaguchi, Jinwook Chung
    • Journal Title

      Analytical Sciences 24・4

      Pages: 547-550

    • Peer Reviewed
  • [Journal Article] Excited-State Structure and Dynamics of 1, 3, 5-Tris(phenylethynyl)benzene as Studied by Raman and Time-Resolved Fluorescence Spectroscopy2007

    • Author(s)
      Tomonori Nomoto, Haruko Hosoi, Tatsuya Fujino, Tahei Tahara, Hiro-o Hamaguchi
    • Journal Title

      Journal of Physical Chemistry A 111・15

      Pages: 2907-2912

    • Peer Reviewed
  • [Journal Article] Picosecond Time-Resolved Fluorescence Study on Solute-Solvent Interaction of 2-Aminoquinoline in Room-Temperature Ionic Liquids: Aromaticity of Imidazolium-Based Ionic Liouids2007

    • Author(s)
      Koichi Iwata, Minoru Kakita, Hiro-o Hamaguch
    • Journal Title

      Journal of Physical Chemistry B 111・18

      Pages: 4914-4919

    • Peer Reviewed
  • [Journal Article] Intra- and intermolecular vibrational energy transfer in tungsten carbonyl complexes W(CO)5(X) (X=CO, CS, CH3CN, and CD3CN)2007

    • Author(s)
      Motohiro Banno, Koichi Iwata, Hiro-o Hamaguchi
    • Journal Title

      Journal of Chemical Physics 126・20

      Pages: 204501

    • Peer Reviewed
  • [Journal Article] Ultrabroadband (>2000cm-1) multiplex coherent anti-Stokes Raman scattering spectroscopy using a subnanosecond supercontinuum light source2007

    • Author(s)
      Masanari Okuno, Hideaki Kano, Pilippe Leproux, Vincent Courdec, Hiro-o Hamaguchi
    • Journal Title

      Optics Letters 32・20

      Pages: 3050-3052

    • Peer Reviewed
  • [Journal Article] Local Structure Formation in Alkyl-imidazolium-Based Ionic Liquids as Revealed by Linear and Nonlinear Raman Spectroscopy2007

    • Author(s)
      Koichi Iwata, Hajime Okajima, Satyen Saha, Hiro-o Hamaguchi
    • Journal Title

      Accounts of Chemical Research 40・11

      Pages: 1174-1181

    • Peer Reviewed
  • [Journal Article] Behaviors of the "Raman Spectroscopic Signature of Life" in Single Living Fission Yeast Cells Under Different Nutrient, Stress, and Atmospheric Conditions2007

    • Author(s)
      Yu-San Huang, Takeshi Nakatsuka, Hiro-o Hamaguchi
    • Journal Title

      Applied Spectroscopy 61・12

      Pages: 1265-1412

    • Peer Reviewed
  • [Journal Article] Supercontinuum Dynamically Visualizes a Dividing Single Cell2007

    • Author(s)
      Hideaki Kano, Hiro-o Hamaguchi
    • Journal Title

      Analytical Chemistry 79・23

      Pages: 6875-6877

    • Peer Reviewed
  • [Presentation] Can Raman Spectroscopy Measure and Quantify Life?2009

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      Physics of Qunatum Electronics Conference 2009
    • Place of Presentation
      Snowbird
    • Year and Date
      20090000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The electron transfer processes in solutions and microparticles studied by femtosecond time-resolved near-IR spectroscopy2008

    • Author(s)
      Tomohisa Takaya, Hiro-o Hamaguchi, Koichi Iwata
    • Organizer
      16th International Symposium of SNU-UT-NTU Chemistry Department
    • Place of Presentation
      Seoul
    • Year and Date
      20080200
  • [Presentation] Recent Advances in Raman Spectroscopy: From a Molecule to a Living Cell2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      Trombay Symposium on Radiation & Photochemsitry
    • Place of Presentation
      Pune
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Liner and Non-Linear Raman Molecular Imaging of Living Cells2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      European Conference on CARS microscopy
    • Place of Presentation
      Igls
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular Near-field Effect in Resonance Hyper-Raman Scattering and Detection of Ensembles of Single Molecules2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      The 17th International Workshop on Laser Physics
    • Place of Presentation
      Trondheim
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] New possibilities of Raman spectroscopy: Detection of ensembles of single molecules and probing the viability of single living cells2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      The 21st International Conference on Raman Spectroscopy
    • Place of Presentation
      London
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] CARS Spectroscopic Imaging Using a Supercontinuum Light Source2008

    • Author(s)
      Hideaki Kano, Hiro-o Hamaguchi
    • Organizer
      The 21st International Conference on Raman Spectroscopy
    • Place of Presentation
      London
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Raman Spectroscopy and Molecular Imaging of Living Cells2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      The 14th European Congress on Molecular Spectroscopy
    • Place of Presentation
      Opatija
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Low Frequency Multi- Channel Raman Spectroscopy with an Iodine Vapor Filter2008

    • Author(s)
      Hajime Okajima, Hiro-o Hamaguchi
    • Organizer
      Federation of Analytical Chemistry and Spectroscopy Societies
    • Place of Presentation
      Reno
    • Year and Date
      20080000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Linear and Non-linear Raman Spectroscopy and Molecular Imaging of Bioactivities in Living Cells2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      Laser Applications in Life Sciences 2008
    • Place of Presentation
      Taipei
    • Year and Date
      2008-12-04
  • [Presentation] High-speed CARS imaging in the fingerprint region by multiplex CARS microspectroscopy2008

    • Author(s)
      Masanari Okuno, Kotatsu Bito, Hideaki Kano, Pilippe Leproux, Vincent Courdec, Hiro-o Hamaguchi
    • Organizer
      日本分光学会年次講演会・シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2008-11-21
  • [Presentation] Molecular Spectroscopic Imaging Using a Supercontinuum Light Source2008

    • Author(s)
      Hideaki Kano, Hiro-o Hamaguchi
    • Organizer
      International Workshop on Nonlinear wave-mixing for laser technology
    • Place of Presentation
      千葉
    • Year and Date
      2008-07-18
  • [Presentation] Raman Spectroscopy: Its Width and Depth.2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      3rd International SAOT-Workshop
    • Place of Presentation
      Erlangen
    • Year and Date
      2008-05-23
  • [Presentation] The "Raman Spectroscopic Signature of Life" And Viability Imaging of Single Living cells2008

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      Focus on Microscopy
    • Place of Presentation
      淡路島
    • Year and Date
      2008-04-14
  • [Presentation] Molecular Spectroscopic Imaging Using a White Laser Source2008

    • Author(s)
      Hideaki Kano, Hiro-o Hamaguchi
    • Organizer
      Tokyo International Symposium on Time- and Space-resolved Vibrational Spectroscopy
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-01-24
  • [Presentation] Raman Spectroscopic Signature of Life And Cell Death at the Molecular Level2007

    • Author(s)
      Hiro-o Hamaguchi
    • Organizer
      1Xth International Conference on Laser Applications in Life Sciences
    • Place of Presentation
      Moscow
    • Year and Date
      20070000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Coherent Raman bioimaging using a supercontinuum light source2007

    • Author(s)
      Hideaki Kano
    • Organizer
      International Symposium, TOPICAL PROBLEMS OF BIOPHOTONICS 2007
    • Place of Presentation
      Moscow
    • Year and Date
      2007-08-06

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Published: 2010-06-11   Modified: 2016-04-21  

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