2019 Fiscal Year Final Research Report
Polymer nano assembly for innovative functional controls of biomolecules
| Project/Area Number |
15H01807
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2020-03-31
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| Keywords | イオン性グラフト共重合体 / 高分子電解質複合体 / 核酸 / ペプチド / 脂質膜 / ナノ会合体 |
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| Outline of Final Research Achievements |
Biological macromolecules must fold into native structures to gain functional activities. In living cells, proteins called molecular chaperones mediate productive folding by preventing undesired interactions and aggregation and by facilitating refolding of misfolded macromolecules into their bioactive forms. Inspired by natural molecular chaperones, artificial chaperones that mimic some features of their biological counterparts have been designed. In this study, we showed that ionic graft copolymers having water soluble side chains formed nano inter polyelectrolyte complexes with oppositely charged biomolecules, such as nucleic acids and peptides. The copolymers facilitated their folding and assembly into functional structures to enhance their functions and activities.
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| Free Research Field |
生体機能性材料
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| Academic Significance and Societal Importance of the Research Achievements |
生体高分子の構造形成を添加成分で制御する試みは内外で行われているが、イオン性相互作用に注目した例は、我々のグループが独自に開始し先進的である。特に、複数の構造間の転移や多分子からなるメゾスケールにおよぶ組織化を介した機能創発まで睨みつつ制御した例は、他に皆無に等しい。一方、機能性核酸やペプチドは、医薬、ナノバイオ領域で一層の注目を浴びており、本研究の成果はその発展に貢献すると考えている。また、脂質は医薬、食品から化粧品まで広く応用されているが、その多くは脂質小胞を利用したものであり、新たに創成された二次元薄膜の脂質シートおよびその動的構造制御法は、脂質の医工学応用等にも様々な可能性を秘める。
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