2018 Fiscal Year Final Research Report
Development of novel biomarkers on exercise, stress and aging and their functional analyses
| Project/Area Number |
15H01833
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied health science
|
|---|
| Research Institution | Waseda University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
秋本 崇之 早稲田大学, スポーツ科学学術院, 教授 (00323460)
|
|---|
| Project Period (FY) |
2015-04-01 – 2019-03-31
|
| Keywords | 翻訳動態 / リボソームプロファイリング / 運動 / 骨格筋 / 老化 / 遺伝子ネットワーク |
|---|
| Outline of Final Research Achievements |
Using aging-model mice and young mice together with acute exercise model mice and endurance exercise model mice, grobal mRNA sequence and ribosome profiling were performed using next-generation sequencer. Changed variables and factors based on the analyses were further confirmed by real-time PCR and western blotting, and messages and proteins were quantified. In addition, gene network analyses were performed to capture changes at the system level of skeletal muscle, and found interesting genes and their potentially new functions.
|
| Free Research Field |
予防医学
|
| Academic Significance and Societal Importance of the Research Achievements |
運動や身体活動を担う骨格筋は総重量が生体の約50％にも達し、骨格筋で転写される骨格筋由来の分子は他の組織由来の分子に比べ多量に存在すると考えられる。老化モデルマウスや運動負荷マウスの骨格筋で網羅的に解析されてリストアップされた遺伝子は生活習慣病や老化の予防に役立つ可能性が高く、本研究ではバイオインフォマティクス的手法を用いて基礎研究レベルでの解析が行われ、新知見が得られた。
|
