Creation of new genome science using chemical tools to precisely detect modification and damage of human genome

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02189
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: National Institute for Materials Science
• Principal Investigator: Komiyama Makoto 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, NIMS招聘研究員 (50133096)
• Co-Investigator(Kenkyū-buntansha): 須磨岡 淳 東京工科大学, 工学部, 教授 (10280934) ; 徐 岩 宮崎大学, 医学部, 教授 (40506763)
• Co-Investigator(Renkei-kenkyūsha): SHIGI Narumi 物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, NIMS特別研究員 (00396780) ; Tanaka Makiko 電気通信大学, 大学院情報理工学研究科, 助教 (90397703) ; Rajendran Arivazhagan 京都大学, エネルギー理工学研究所, 講師 (90723122)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ゲノム / PNA / インベージョン / ゲノム断片分離 / エピゲノム

Outline of Final Research Achievements

We already developed super artificial restriction enzyme. In this research, we added affinity separating ability to this tool, and constructed the second- generation super artificial restriction enzyme. By modifying biotin to PNA which is a constituent of super restriction enzyme, we successfully extracted desired DNA fragments of several thousand base pairs in high purity and high yield from the human genome with 3 billion base pairs. Furthermore, by combining this biotin-modified PNA with S1 nuclease, we also succeeded in extracting desired DNA fragments from the human genome in one pot.

Free Research Field

化学