Structural study of light-driven sodium pump

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02391
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Kandori Hideki 名古屋工業大学, 工学(系)研究科(研究院), 教授 (70202033)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ロドプシン / ナトリウムポンプ / 光反応サイクル / 結晶構造解析 / 赤外分光

Outline of Final Research Achievements

Although it was believed that rhodopsins cannot pump non-proton cations, we discovered a light-driven sodium pump rhodopsin in 2013. This project aims at revealing the structure of sodium pump rhodopsins. In collaboration with Nureki Lab of Univ. Tokyo, we successfully crystalized a light-driven sodium pump KR2, and proposed a model of sodium pumping. We also performed light-induced difference FTIR spectroscopy, such as low-temperature, time-resolved, and attenuated total reflection (ATR) methods, to sodium pump rhodopsins, and obtained important structural information. We reported more than 50 articles in Nature, Nature Commun., JACS, Angew., PNAS, JPC Letters and JBC during the project period.

Free Research Field

生物物理学