Regulation of radiation-induced DNA damage repair through chromatin remodeling

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02819
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: Kobayashi Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
• Co-Investigator(Kenkyū-buntansha): 林 幾江 広島大学, 医歯薬保健学研究科(歯), 助教 (00346503)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA損傷 / 放射線 / DNA修復 / クロマチン / クロマチンリモデリング / 複製ストレス

Outline of Final Research Achievements

As the importance of chromatin remodeling factors has been reported in radiation-induced DNA double-strand breaks (DSBs) damage responses, we investigated to identify chromatin remodeling factors, which could function in DSB damage responses,using immunoprecipitation/proteomics analysis. As a results, we identified several candidates of chromatin remodeling-related factors. Among of them, we found that nucleolin could function in ATR activation and homologous recombination, which suggests that nucelolin might be an important factor for replication stress-related cellular responses.

Free Research Field

放射線生物学