Study of the mechanisms of radiation-induced chromosome abnormalities

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02821
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Hiroshima University
• Principal Investigator: Tashiro Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
• Co-Investigator(Kenkyū-buntansha): 堀越 保則 広島大学, 原爆放射線医科学研究所, 助教 (00719429)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 放射線被ばく / 損傷ゲノム / 細胞核構造 / ゲノム修復 / 染色体異常

Outline of Final Research Achievements

In this study, I have published papers concerning the mechanism of RAD51 focus formation involving nuclear proteins such as LaminB, a nuclear lamina related protein, after induction of DNA damage. I have also reported the result of the long time follow-up of a patient receiving radio-iodine treatment using cytogenetical analysis and estimation of radiation effect by a cardiac CT scan using immunofluorescence analysis using anti-gH2AX antibodies. I have also performed a dynamic structural analysis of radiation-induced nuclear domains using super-resolution microscopy. We found that the shape of RAD51 foci is changed by ionizing irradiation. We are now preparing a manuscript concerning the effect of ionizing radiation on the fine structure of radiation-induced nuclear domains.

Free Research Field

放射線生物学