2017 Fiscal Year Final Research Report
Mechanism of cluster DNA damage induction and its repair processes in vivo
| Project/Area Number |
15H02823
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology (2016-2017)
Japan Atomic Energy Agency (2015) |
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Principal Investigator |
Yokoya Akinari 国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 東海量子ビーム応用研究センター, 上席研究員(定常) (10354987)
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| Co-Investigator(Kenkyū-buntansha) |
渡辺 立子 国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 東海量子ビーム応用研究センター, 主幹研究員(定常) (10360439)
秋光 信佳 東京大学, アイソトープ総合センター, 教授 (40294962)
岡 壽崇 東北大学, 高度教養教育・学生支援機構, 助教 (70339745)
鵜飼 正敏 東京農工大学, その他の研究科, 助教授 (80192508)
福永 久典 東北大学, 大学病院, 医員(研修医) (50781267)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII KENTARO 量子科学技術研究開発機構, 高崎量子応用研究所・東海量子ビーム応用研究センター, 上席研究員 (00360404)
HATTORI YUYA 東京工業大学, 工学院・システム制御系, 助教 (30709803)
NOGUCHI MIHO 量子科学技術研究開発機構, 高崎量子応用研究所・東海量子ビーム応用研究センター, 主幹研究員 (40455283)
IZUMI YUDAI 広島大学, 放射光科学研究センター, 助教 (20595772)
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| Research Collaborator |
Hervé du Penhoat Marie-Anne Sorbonne Universite's - UPMC Univ Paris 06, IMPMC
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | クラスターDNA損傷 / EGFP / ライブセルイメージング / 修復速度 / 放射線トラックエンド / 低エネルギー二次電子 / 水和前電子 / 塩基損傷 |
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| Outline of Final Research Achievements |
Repair process of clustered DNA damage induced by X-irradiation was investigated using EGFP-plasmid DNA transfected into non-irradiated mammalian cells. The repair kinetics of the irradiated plasmids in the cells visualized as the EGFP fluorescence emission indicates that the damage was hardly repaired. Hydrated deoxyribose (dR) films were irradiated with soft X-rays. Yields of desorbed ions from the films were significantly suppressed by water. Molecular dynamics calculation indicates that the fast proton transfer from dR to the hydrated water might cause the suppression. Furthermore, dynamics of low energy secondary electrons around DNA molecule was investigated using a dynamic Monte Carlo code. The results show that pre-hydrated electrons are formed over a few nm from the parent cations and result in additional base lesions, which may be finally converted into a harmful double strand break by base excision processes.
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| Free Research Field |
放射線生物物理学
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