New development of clarification and control on mechanism of neurogenesis of fluorescent enteric neurons responding to stimuli.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Nara Medical University
• Principal Investigator: Takaki Miyako 奈良県立医科大学, 医学部, 研究員 (00033358)
• Co-Investigator(Kenkyū-buntansha): 國安 弘基 奈良県立医科大学, 医学部, 教授 (00253055) ; 中井 淳一 埼玉大学, 理工学研究科, 教授 (80237198)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: リハビリテーション医学 / Caイメージング / セロトニン４受容体 / 腸壁内神経 / 神経再生・新生 / 腸管切離吻合モデル / GCaMPトランスジェニックマウス / ２光子顕微鏡

Outline of Final Research Achievements

The reconstruct of impaired enteric neural circuits at the thick granulation tissue at anastomosis in small intestine of H-line: Thy1 promoter GFP mouse was observed by two-photon microscopy a week after drinking a 5-HT4-receptor agonist, mosapride citrate (MOS) 100 microM solution.
In Thy1-promoter YFP mouse after gut transection and anastomosis, the fetal brain-derived neural stem cells (NSC) with red fluorescent cell linker were transplanted from the tail vein. 5-HT4 receptor-mediated facilitation of neurogenesis was confirmed by clear three-dimensional in vivo imaging of newborn enteric neurons generated from enteric neural progenitors (host NSC; green fluorescence) and those from transplant NSC (red fluorescence). The facilitating effect of MOS and the distribution of new neurons were similar between the transplant and host NSC. Finally, though preliminary, we succeeded in in vivo Ca2+ imaging of myenteric neuronal activity in the normal ileum of Thy1-G6-mCherry transgenic mouse.

Free Research Field

生理学