electrochemical control of mas transfer and single cell analysis by multifunctional nanopipet

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03542
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nano/Microsystems
• Research Institution: Tohoku University
• Principal Investigator: Shiku Hitoshi 東北大学, 工学研究科, 教授 (10361164)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 走査型プローブ顕微鏡 / 液液界面 / 遺伝子発現解析 / 組織モデル / 胚性幹細胞 / 分化誘導 / イオンコンダクタンス / 電気化学

Outline of Final Research Achievements

Recently, single-cell analysis has been developed to achieve highly sensitive and high-throughput system. Large-scale data analysis of gene expression profiles for actual tissue samples of colorectal cancer patients. However, in the present stage, expensive cell sorter is required to perform single-cell analysis of tissue samples. And the cell sorting process lose information about the original location of the single cells in the tissue samples. We have developed a single-cell analytical tool based on scanning probe microscopy to obtain high-resolution topographic images of tissue model. Sequentially, the SPM system allows to precisely collect a targeted single-cell or a part of cytosol sample from a single-cell for high-throughput gene expression analysis.

Free Research Field

分析化学