Correlation between property and fluctuation of the lipid membranes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03824
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Chiba University
• Principal Investigator: Fujinami Masanori 千葉大学, 大学院工学研究院, 教授 (50311436)
• Co-Investigator(Kenkyū-buntansha): 豊田 太郎 東京大学, 総合文化研究科, その他 (80422377)
• Co-Investigator(Renkei-kenkyūsha): NOMOTO Tomonori 千葉大学, 大学院工学研究院, 助教 (00510520)
• Research Collaborator: CHIARI Luca 千葉大学, 大学院工学研究院, 特任助教 ; WATAHIKI Yasuhito 千葉大学, 大学院融合理工学府, 大学院生 ; TAKAHASHI Masahiro 千葉大学, 大学院工学研究科, 大学院生 ; FUJII Takuya 千葉大学, 大学院工学研究科, 大学院生 ; KARASAWA Yuichiro 千葉大学, 大学院工学研究科, 大学院生 ; IKO Satoshi 千葉大学, 大学院工学研究科, 大学院生 ; TAKEI Tomohiko 千葉大学, 大学院工学研究科, 大学院生 ; UEMOTO Shoko 千葉大学, 大学院工学研究科, 大学院生 ; GOTO Kazuma 千葉大学, 大学院工学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 分析科学 / 生体物理 / 生体分子 / 生体膜 / 表面・界面物性 / 界面活性分子

Outline of Final Research Achievements

In the current study, the membrane tension and the diffusion coefficients of free-standing black lipid membranes (BLMs) were observed by systematic control of the cholesterol concentration and the osmotic pressure with the laser induced surface deformation (LISD) and fluorescence recovery after photobleaching (FRAP) techniques. When the osmotic pressure was raised, both the membrane tension and diffusion coefficients increased as well. On the other hand, when the cholesterol concentration was raised, the membrane tension increased whereas the diffusion coefficient decreased. The results are not only important as the quantitative evaluations about relations between membrane tension and fluidity, but also important as the fundamental properties of lipid bilayers with natural fluctuations and perturbative deformation that were unavailable before.

Free Research Field

分析化学