2017 Fiscal Year Final Research Report
Development of novel diagnostics for hematological malignancy on basis of the exosome analysis
| Project/Area Number |
15H04303
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnostics
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大屋敷 純子 東京医科大学, 医学部, 教授 (20191950)
梅津 知宏 東京医科大学, 医学部, 講師 (40385547)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | miRNA / 細胞外小胞 / 骨髄異形成症候群 / 骨髄間質細胞 |
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| Outline of Final Research Achievements |
Bone marrow stromal cells (BMSCs) play an important role in bone marrow environment. Recent evidences suggest that extracellular vesicles (EVs) act as a mediator of cell-cell interaction in hematologic neoplasms. To clarify the possible association between the cargo of BMSC-EVs and disease severity, we performed miRNA profiling in BMSC-EVs derived from MDS patients.
BMSCs from 29 MDS patients were obtained by classical adhesion methods. We tentatively separated MDS patients into two groups according to the IPSS-R: low-risk and high-risk group. We found that a subset of EV-miRNA was differentially expressed between the two groups. Among them, we in particular focus on EV-miR-101: the expression level was significantly lower in high-risk MDS. Cell-cell communication via EV-miR-101 may play in part some role between BMSC and MDS cells. Our study shed light on the biological relevance of BMSC-EV and EV-miRNA in MDS bone marrow microenvironment.
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| Free Research Field |
臨床腫瘍学
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