Elucidation of the chemoresistance in pancreatic cancer and development of its tumor stroma targeting ADCs

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04316
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: National Cancer Center Japan
• Principal Investigator: Yasunaga Masahiro 国立研究開発法人国立がん研究センター, 先端医療開発センター, ユニット長 (80450576)
• Research Collaborator: Matsumura Yasuhiro 国立研究開発法人国立がん研究センター, 先端医療開発センター, 分野長 (90209619) ; Manabe Shino 独立行政法人理化学研究所, 伊藤細胞制御化学, 専任研究員 (60300901)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗体 / ADC / 膵臓がん / がん間質 / 間質バリア / DDS / 分子イメージング / ドラッグイメージング

Outline of Final Research Achievements

(1) Pancreatic cancer model mice with a strong desmoplastic reaction similar to human pancreatic cancer was prepared. (2) We succeeded in developing a new drug imaging method by combining robot-controlled ESI (Electrospray ionization) method and an LC-MS apparatus. It has become possible to observe drug delivery within the tumor tissue with high sensitivity. (3) a) Anti-fibroblast / b) stellate and parietal cell / c) blood-vessel / d) blood and lymphoid vessel antibodies showed good tumor-stroma targeting within the tumor tissues. Regarding antibody-drug conjugates (ADCs), they were newly-produced and therapeutic experiments were conducted in the model of (1). A strong antitumor effect was observed in the order of a)b)c)d).(4) Anti-tissue factor/fibrin ADCs also showed therapeutic effect in the same mouse model.

Free Research Field

総合生物