Investigating phosphoregulation of meiotic recombination using superresolution microscopy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04328
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kyoto University
• Principal Investigator: Carlton Peter 京都大学, 生命科学研究科, 准教授 (20571813)
• Research Collaborator: SATO CARLTON Aya
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 減数分裂 / 線虫 / 高解像度顕微鏡 / 染色体

Outline of Final Research Achievements

In this grant period we investigated control of meiotic prophase chromosome dynamics by phosphorylation in C. elegans. Our starting point was the phosphatase PPH-4.1, which we had previously identified as a critical regulator of at least 4 critical chromosome dynamics events. We identified SYP-1, a member of the synaptonemal complex, as an important phosphoprotein in meiosis. SYP-1 phosphorylation is required for correct chromosome segregation, acting to specify the region in which chromosome cohesion will be lost in the first meiotic division. Additionally, our data shows that SYP-1 phosphorylation is required to recruit Polo-like kinase to the chromosome central region, as well as to promote the correct distribution of genetic crossovers on chromosomes.

Free Research Field

減数分裂においての染色体ダイナミクス