Identification and characterization of interactions between genomic regions using the locus-specific chromatin immunoprecipitation technology

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04329
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Fujii Hodaka 大阪大学, 微生物病研究所, 招へい教授 (30302665)
• Co-Investigator(Kenkyū-buntansha): 藤田 敏次 大阪大学, 微生物病研究所, 招へい准教授 (10550030)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: クロマチン / エピジェネティクス / ゲノム生化学 / enChIP / iChIP / 核内三次元構造

Outline of Final Research Achievements

Using the locus-specific chromatin immunoprecipitation (ChIP) technology, we identified many genomic regions interacting with the β-globin gene 5'HS5 region constitutively or inducibly by induction of differentiation with sodium butyrate (NaB). Expression of a group of genes in the vicinity of the interacting sites was induced by NaB stimulation, suggesting that NaB-induced interactions between genomic regions is involved in regulation of the cooperative gene expression program. In addition, in an effort to elucidate molecular mechanisms of dynamic regulation of interferon-inducible genes, we developed enChIP systems using different dCas9 orthologues and tags. We further showed that the yields of the novel enChIP systems are sufficiently high for their practical use.

Free Research Field

分子生物学、エピジェネティクス、クロマチン