2017 Fiscal Year Final Research Report
Post-transcriptional gene expression regulation via mRNA surveillance system
| Project/Area Number |
15H04331
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | NMD / mRNA分解 / mRNA代謝 / 翻訳終結 / 遺伝性疾患 / がん |
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| Outline of Final Research Achievements |
The nonsense-mediated mRNA decay (NMD) pathway it acts to selectively identify and degrade mRNAs that contain a premature translation termination codon (PTC), and hence reduce the accumulation of potentially toxic truncated proteins. SMG1, a member of the PIKK (phosphoinositide 3-kinase related kinases) family, plays a critical role in NMD. According to prevailing models, NMD begins by the assembly of the SURF (SMG1-UPF1-eRF1-eRF3) complex at the ribosome, followed by UPF1 activation by additional factors such as UPF2 and UPF3.In present study we demonstrated that the interaction between human UPF2 and eukaryotic release factor 3 (eRF3). In addition, we find that UPF2 associates with SURF and ribosomes in cells, in an UPF3-independent manner. In addition, we showed that the existence of a complex comprising SMG1, UPF1 and DHX34, with functioning as a potential scaffold for UPF1 and SMG1.
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| Free Research Field |
分子生物学
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