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2017 Fiscal Year Final Research Report

New Frontier of Photosynthesis Research by Using the Cryogenic Optical Microscope

Research Project

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Project/Area Number 15H04356
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

Shibata Yutaka  東北大学, 理学研究科, 准教授 (20300832)

Co-Investigator(Kenkyū-buntansha) 野口 巧  名古屋大学, 理学研究科, 教授 (60241246)
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords共焦点顕微鏡 / 極低温 / 単一分子分光 / ステート遷移
Outline of Final Research Achievements

By using the developed cryogenic microscope system, intracellular inhomogeneous distributions of photosynthetic proteins of Chlamydomonas, a model organism for the photosynthesis study, were observed. The analysis clarified the extent of co-localizations of photosystem proteins and light-harvesting antenna proteins. This results demonstrated that antenna proteins actually shuttles between the two photosystem proteins upon state transition, which is a regulation mechanism of excitation balance of the two photosystems.
A system enabling scanning of the excitation wavelength was developed. By using the system, the fluorescence excitation spectra of single photosynthesis proteins were obtained for the first time. Information about the excitation-energy flow within the protein was obtained.

Free Research Field

光生物学、光合成の生物物理学

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Published: 2019-03-29  

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