Elucidation of translational regulatory mechanism by RNA-protein complex

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04358
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Funatsu Takashi 東京大学, 大学院薬学系研究科(薬学部), 教授 (00190124)
• Co-Investigator(Renkei-kenkyūsha): OKABE Kohki 東京大学, 大学院薬学系研究科, 助教 (20455398) ; IIZUKA Ryo 東京大学, 大学院薬学系研究科, 助教 (90541954)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: １分子計測（SMD） / ナノバイオ / 蛋白質

Outline of Final Research Achievements

1. Fluorescently labeled miRNA precursors were microinjected into a cell. They were matured by endogenous biosynthetic pathway and they were incorporated into RISC. Single-molecule imaging technique revealed that miRNAs move on actin filaments.
2. Localization of mRNAs labeled with Cy5-linear antisense DNA probe was investigated under the stress conditions. Super-resolution microcopy revealed that mRNAs are not uniformly present in the stress granule (SG) but are accumulated to form high density regions with a diameter of about 100 nm.
3. The lifetimes of translational arrest of SecM mutants, whose amino acids outside ribosome were partially truncated, were investigated. Amino acid residues 57-73 and 57-98 contributed to the stabilization of translational arrest.

Free Research Field

生物物理学