2017 Fiscal Year Final Research Report
Elucidation of energy conversion mechanism of a Na+-transporting rotary ATPase
| Project/Area Number |
15H04366
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
IINO RYOTA 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (70403003)
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| Research Collaborator |
IIDA Tatsuya
MURATA Takeshi
UENO Hiroshi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 分子モーター / １分子計測 |
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| Outline of Final Research Achievements |
Enterococcus hirae V-ATPase (EhVoV1) is a rotary molecular motor which functions as a sodium ion pump across the biological membrane. The hydrophilic potion EhV1 converts chemical energy of ATP hydrolysis into unidirectional mechanical rotation. To understand chemo-mechanical coupling mechanism of Enterococcus hirae V1 (EhV1), we conducted single-molecule rotation assay using 40-nm gold nanoparticle as a low-load probe. We found that 120° steps per ATP hydrolysis were further divided into 40° and 80° substeps. Based on the results obtained in this study and insight of the previous structural analysis by X-ray crystallography, we proposed a complete chemo-mechanical coupling scheme of EhV1.
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| Free Research Field |
生物物理学
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