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2017 Fiscal Year Final Research Report

Elucidation of energy conversion mechanism of a Na+-transporting rotary ATPase

Research Project

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Project/Area Number 15H04366
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

IINO RYOTA  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (70403003)

Research Collaborator IIDA Tatsuya  
MURATA Takeshi  
UENO Hiroshi  
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords分子モーター / 1分子計測
Outline of Final Research Achievements

Enterococcus hirae V-ATPase (EhVoV1) is a rotary molecular motor which functions as a sodium ion pump across the biological membrane. The hydrophilic potion EhV1 converts chemical energy of ATP hydrolysis into unidirectional mechanical rotation. To understand chemo-mechanical coupling mechanism of Enterococcus hirae V1 (EhV1), we conducted single-molecule rotation assay using 40-nm gold nanoparticle as a low-load probe. We found that 120° steps per ATP hydrolysis were further divided into 40° and 80° substeps. Based on the results obtained in this study and insight of the previous structural analysis by X-ray crystallography, we proposed a complete chemo-mechanical coupling scheme of EhV1.

Free Research Field

生物物理学

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Published: 2019-03-29  

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