Mechanisms of mRNA alternative splicing and ABA-responsive phosphosignaling

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04383
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Umezawa Taishi 東京農工大学, (連合)農学研究科(研究院), 准教授 (70342756)
• Co-Investigator(Kenkyū-buntansha): 平山 隆志 岡山大学, 資源植物科学研究所, 教授 (10228819) ; 坂田 洋一 東京農業大学, 生命科学部, 教授 (50277240)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アブシシン酸 / リン酸化 / スプライシング / プロテインキナーゼ / 乾燥耐性 / シロイヌナズナ

Outline of Final Research Achievements

Abscisic acid (ABA) is a major phytohormone which regulates stress responses in plants. It had been demonstrated that protein phosphorylation is important in ABA signal transduction pathway, and we found several mRNA splicing factors as phosphoproteins in response to ABA. To elucidate splicing events in response to ABA, we performed RNA-seq analysis using Arabidopsis WT and srk2dei mutant, and identified over 170 splice variants were regulated by ABA and SnRK2. We are going to functional analysis of splice variants in ABA signaling, and RNA-seq analysis using Arabidopsis seeds in the same way.

Free Research Field

植物分子生物学