Elucidation of physiological roles and regulation of the plasma membrane H+-ATPase

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04386
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Kinoshita Toshinori 名古屋大学, 理学研究科(WPI), 教授 (50271101)
• Co-Investigator(Renkei-kenkyūsha): KOHCHI Takayuki 京都大学, 生命科学研究科, 教授 (40202056) ; ISHIZAKI Kimitsune 神戸大学, 理学(系)研究科, 准教授 (00452293) ; OHTA Hiroyuki 東京工業大学, 生命理工学研究科, 教授 (20233140)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 植物 / 細胞膜プロトンポンプ / 光合成 / 養分吸収 / 植物ホルモン / リン酸化

Outline of Final Research Achievements

The plasma membrane (PM) H+-ATPase in plants mediates numerous important physiological processes, such nutrient uptake in roots, stomatal opening, and phloem loading. However, the molecular and regulatory mechanisms of this enzyme is still remains unknown. In this study, we investigated regulatory mechanism and physiological roles of PM H+-ATPase in plants and found that PM H+-ATPase is regulated photosynthesis-dependent manner in Arabidopsis and Klebsormidium, and that PM H+-ATPase in rice roots are activated by one of nutrient silicon, which has important role for the stress tolerant in rice. Furthermore, we found that plant hormones brassinosteroid and auxin activate PM H+-ATPase in etiolated hypocotyl in Arabidopsis through their receptors, BRI1 and TIR1, respectively. These results provide important information for understanding molecular mechanism and physiological role of PM H+-ATPase in plant growth.

Free Research Field

植物分子生理学