2017 Fiscal Year Final Research Report
Identification of a sex-determining gene in Emu
| Project/Area Number |
15H04401
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kuroiwa Asato 北海道大学, 理学研究院, 教授 (20372261)
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| Co-Investigator(Kenkyū-buntansha) |
河野 友宏 東京農業大学, 応用生物科学部, 教授 (80153485)
松田 洋一 名古屋大学, 生命農学研究科, 教授 (70165835)
西田 千鶴子 北海道大学, 理学(系)研究科(研究院), 助手 (50106580)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | W染色体 / 性決定機構 |
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| Outline of Final Research Achievements |
A sex of birds is genetically determined. However, the female sex-determining gene on the W chromosome has been not identified. To identify a novel gene located on the W which function on the ovary differentiation in emu, I performed three approaches: 1) cell culture, chromosome preparation, micro dissection, DNA purification of Z and W chromosome, 2) identification of sex-determining stage of embryo, 3) mRNA-seq analysis in gonads. I identified candidates genes for sex-determination located on the Z and W chromosomes. Furthermore,transcriptional variants of DMRT1 in emu were detected.
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| Free Research Field |
生殖発生学
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