2017 Fiscal Year Final Research Report
Elucidation of the working principle of cells based on high dimensional phenotyping
| Project/Area Number |
15H04402
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ohya Yoshikazu 東京大学, 大学院新領域創成科学研究科, 教授 (20183767)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Saccharomyces cerevisiae / morphology / CalMorph / phenotyping |
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| Outline of Final Research Achievements |
It is one of the central issues of genetics to unravel the principle of motion of an organism by genetic information, that is, the principle of how genotype "cause" is linked to phenotype "result". In order to clarify the relationship between genotype and phenotype, we used the excellent genetic tool of unicellular eukaryotic budding yeast (S. cerevisiae) and used cell images obtained with fluorescence microscope. We analyzed the images with image processing system CalMorph and found that there is a close relationship between gene function and morphological phenotype. This was achieved by quantitatively analyzing high dimensional phenotype from two viewpoints including morphological profiling and morphological abnormality. Using that relationship, we succeeded in identifying intracellular targets of drugs.
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| Free Research Field |
Functional Genomics
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