Variety and regulation of lanthanide-dependent methanol dehydrogenases

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04476
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Okayama University
• Principal Investigator: Tani Akio 岡山大学, 資源植物科学研究所, 准教授 (00335621)
• Co-Investigator(Renkei-kenkyūsha): MITSUI Ryoji 岡山理科大学, 理学部生物科学科, 教授 (60319936) ; NAKAGAWA Tomoyuki 岐阜大学, 応用生物科学部, 教授 (70318179)
• Research Collaborator: LV Haoxin 岡山大学, 資源植物科学研究所
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: メタノール / ランタノイド / メチロトローフ

Outline of Final Research Achievements

We genetically analyzed the function of multiple methanol dehydrogenase (MDH) -like genes in Methylobacterium species. It was found that Ca-dependent MDH, MxaF and Ln-dependent MDH, XoxF1 were necessary for the growth on methanol and ethanol. Another alcohol dehydrogenase is involved in propanol oxidation. In addition, genetic analysis on MxbD that acts as lanthanide-switch for the expression of mxaF and xoxF has been done. MxbD is necessary for mxaF expression but not for xoxF expression, and spontaneous mutation in HAMP domain in the protein releases the repression of mxaF expression by the presence of XoxF.

Free Research Field

応用微生物学