Linking novel bioactive compounds to their targets and pathways using chemical genomics and morphological profiling

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04483
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Boone Charles 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (70601342)
• Co-Investigator(Kenkyū-buntansha): 大矢 禎一 東京大学, 大学院新領域創成科学研究科, 教授 (20183767)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: chemical genomics / morphological profiling / yeast / target identification

Outline of Final Research Achievements

Combining chemical genomics profiling based on genetic interactions and morphological profiling using a sophisticated image-processing program, CalMorph, we developed a multi-dimensional strategy for linking novel bioactive compounds to their targets and pathways. We established a high-throughput chemical-genetic screening platform to functionally annotate large compound collections in a rapid and systematic manner using next generation sequencing. Furthermore, we assembled a computational platform for functionally annotating compounds to specific biological processes and pathways. Finally, we applied our chemical-genomic/morphological profiling pipeline to annotate 13,524 compounds in total by screening seven diverse compound collections including the RIKEN NPDepo library.

Free Research Field

yeast chemical genomics