Development of drug leads targeting fungal glycans

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04496
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Nagoya University
• Principal Investigator: Nakagawa Yu 名古屋大学, 生命農学研究科, 准教授 (90452284)
• Co-Investigator(Kenkyū-buntansha): 竹腰 清乃理 京都大学, 理学研究科, 教授 (10206964) ; 相田 美砂子 広島大学, 理学研究科, 教授 (90175159)
• Co-Investigator(Renkei-kenkyūsha): OJIKA Makoto 名古屋大学, 大学院生命農学研究科, 教授 (50152492)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗生物質 / 糖鎖 / pradimicin / 分子認識

Outline of Final Research Achievements

This study uncovered the molecular basis of D-mannose (Man) recognition of Pradimicin A (PRM-A) by a combination of X-ray crystallography and solid-state NMR spectroscopy. It is also suggested by binding studies using synthetic oligomannoses that PRM-A could recognize regions in fungal mannans where Man residues at the non-reducing ends are densely located. Based on these findings, we developed two derivatives of PRM-A, PRM-Amide with a less aggregative property and PRM-Azide capable of fluorescently staining fungal mannans.

Free Research Field

天然物化学