Analysis of individual chromosome dynamics in living embryo and establishment of next generation embryo manipulation technique by chromosome transfer

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04605
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: The University of Tokyo
• Principal Investigator: MIZUTANI EIJI 東京大学, 医科学研究所, 特任研究員 (80443034)
• Co-Investigator(Kenkyū-buntansha): 長友 啓明 山梨大学, 大学院総合研究部, 特任助教 (30746813) ; 若山 照彦 山梨大学, 大学院総合研究部, 教授 (40360672)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 染色体操作 / 核移植 / イメージング

Outline of Final Research Achievements

Nuclear transfer technique manipulates the nucleus itself, but attempts to develop technologies to manipulate smaller units, ie chromosomes. We used the suntag-system to label specific chromosomes and succeeded in labeling the Y chromosome in ES cells and showed the possibility of labeling any chromosome. It also showed that chromosomal transfer between live embryos is possible by dispersing the chromosome in embryos. However, we failed to label and manipulate specific chromosomes in embryos at present. In addition, cloned mouse and ntES cell lines can be established from very few cells contained in the urine that can be harvested without harming individuals in the threatened species expected to use chromosomal manipulation.

Free Research Field

発生工学