Dedifferentiated fat (DFAT) cells as a new donor cells for regenerative medicine.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04607
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Nihon University
• Principal Investigator: KANO Koichiro 日本大学, 生物資源科学部, 教授 (80271039)
• Co-Investigator(Kenkyū-buntansha): 沖 嘉尚 日本大学, 生物資源科学部, 助教 (70525667) ; 森友 忠昭 日本大学, 生物資源科学部, 教授 (20239677) ; 伊藤 大介 日本大学, 生物資源科学部, 講師 (40508694) ; 北川 勝人 日本大学, 生物資源科学部, 教授 (50409067)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 成熟脂肪細胞 / 自発的脱分化 / 分化多能性 / 脱分化脂肪細胞 / 獣医再生医学

Outline of Final Research Achievements

When mature adipocytes are subjected to an in vitro dedifferentiation strategy referred to as ceiling culture, these mature adipocytes can revert to a more primitive phenotype and gain cell proliferative ability. We refer to these cells as dedifferentiated fat (DFAT) cells. In the present study, we examined the multilineage differentiation potential of DFAT cells. DFAT cells obtained from adipose tissues of various animals exhibited a fibroblast-like morphology and sustained high proliferative activity. In vitro differentiation analysis revealed that DFAT cells could differentiate into adipocytes, osteoblasts and nerve cells under appropriate culture conditions. These results indicate that DFAT cells represent a type of multipotent progenitor cell. Since DFAT cells can be prepared from a small quantity of adipose tissue, they could facilitate cell-based therapies in small companion Animals such as dogs and cats.

Free Research Field

細胞生物学、発生・再生生物学、再生医学