New approach for studying functional importance of baculovirus nonessential genes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04608
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Insect science
• Research Institution: Hokkaido University
• Principal Investigator: Bando Hisanori 北海道大学, 農学研究院, 教授 (20189731)
• Co-Investigator(Kenkyū-buntansha): 佐藤 昌直 北海道大学, 農学研究院, 助教 (20517693)
• Research Collaborator: TAKA Hitomi ; ISHIBASHI Taiki ; HARADA Naoto ; SAITO Ryo
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 昆虫機能利用 / バキュロウイルス

Outline of Final Research Achievements

In Bombyx mori nucleopolyhedrovirus (BmNPV) genome comprised of 143 genes, more than 60 % of them are nonessential genes for efficient multiplication in Bombyx mori cell line BmN. However, a combination of nonessential genes was found to play a critical role in efficient viral multiplication, suggested essentiality of nonessential genes as a collected gene. A study using reverse genetics identified genetic interactions among nonessential gens in such a combination and emphasized importance of a methodology to advance the reverse combinatorial genetics of BmNPVs; thus we established a platform for BmNPV genome assembly from PCR-amplified DNA fragments using Gibson Assembly for further analysis of the essentiality of nonessential genes and for reconstruction of BmNPV vector system based on logical design.

Free Research Field

応用分子昆虫学