Structural life science of pathogenic bacterial systems targeting animal host extracellular matrix for colonization and infection

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04629
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: HASHIMOTO Wataru 京都大学, 農学研究科, 教授 (30273519)
• Co-Investigator(Kenkyū-buntansha): 三上 文三 京都大学, 農学研究科, 教授 (40135611) ; 丸山 如江 摂南大学, 理工学部, 助教 (90397563)
• Co-Investigator(Renkei-kenkyūsha): MURATA Kousaku 摂南大学, 理工学部, 教授 (90142299)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 病原性細菌 / 細胞外マトリクス / グリコサミノグリカン / フォスフォトランスフェラーゼ系 / ABCトランスポーター / 分解・代謝酵素 / 遺伝子クラスター / X線結晶構造解析

Outline of Final Research Achievements

For colonization and invasion to animal cells, some pathogenic bacteria such as Streptococcus target glycosaminoglycans present in host extracellular matrices. In this study, streptococcal phosphotransferase system involved in the import of a glycosaminoglycan hyaluronan as well as the bacterial degradation/metabolism mechanisms of the fragmented hyaluronan were clarified by molecular and structural biology. A novel ABC transporter was also identified in Streptobacillus to be an importer of fragmented glycosaminoglycans. Based on results obtained here, inhibitors of these bacterial molecular machineries are expected to become promising agents for the bacterial infectious diseases.

Free Research Field

応用微生物学