Analysis of sensing and responding mechanisms for osmotic stress in mammalian cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04643
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: Naguro Isao 東京大学, 大学院薬学系研究科(薬学部), 准教授 (80401222)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 浸透圧ストレス / ASK3 / NFAT5 / ゲノムワイドsiRNAスクリーニング

Outline of Final Research Achievements

Recently, molecules that function in cellular osmotic stress response have been reported to be involved in the regulation of blood pressure, inflammation and immune system. However, the precise molecular mechanism for sensing and transducing osmotic stress in mammalian cells still remains to be elucidated. In this project, we focused on ASK3 and NFAT5, two molecules responding to osmotic stress. With original methodologies, such as genome-wide siRNA screens and specific cross-linking technology, we have elucidated new molecular mechanisms regulating osmotic stress response in mammalian cells.

Free Research Field

物理化学的ストレスの細胞内シグナル伝達機構