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2016 Fiscal Year Annual Research Report

ラマン分光法を用いた,好酸球性食道炎の非生検診断法の開発

Research Project

Project/Area Number 15H04763
Research InstitutionShimane University

Principal Investigator

山本 達之  島根大学, 生物資源科学部, 教授 (60230570)

Co-Investigator(Kenkyū-buntansha) 木下 芳一  島根大学, 医学部, 教授 (30243306)
Project Period (FY) 2015-04-01 – 2018-03-31
Keywordsラマンスペクトル / 好酸球性食道炎 / エオシノフィルペルオキシダーゼ / 内視鏡 / 新規診断法
Outline of Annual Research Achievements

近年患者数が増加している好酸球性食道炎は,内視鏡による確定診断が困難である。現在唯一の確定診断法は,食道粘膜の多数箇所の生検による病理組織診断である。本疾患の患者は,その多くが高齢者であるため,抗血栓薬の服用者が多い。このため,生検による出血が大きな問題となっている。本研究課題では,ラマン分光法による,患者負担が少ない新規診断法の確立を目指す。ラマン分光法は,試料の前処理が一切不要な低侵襲的分光分析法で,5種類の白血球から好酸球の識別ができる。そこで,内視鏡に組み込めるファイバスコープ型ラマンスペクトル測定装置と,好酸球性食道炎の診断が可能なソフトウェアの開発を進める。これらの開発により,ラマン分光法に関する知識が無くても臨床応用可能な新規診断法を確立することが,本研究の目的である。
平成28年度は,内視鏡に組み込み可能な装置の実現に向けて,生体試料のラマン測定に特化した医療ラマン装置の開発と,ヒトから採取した血液から分離した顆粒球のラマン測定を行った、医療ラマン装置は,ラマン分光法の専門家でなくても操作可能な自動測定装置を組み込んだものとした。装置のハードウェアは完成して,ラマン測定可能な状態になったが,自動測定可能なソフトウェアの開発を,年度内に終了することはできなかった。平成29年度のできるだけ早い段階で,自動測定ソフトウェアを装置にインストールして,島根大学医学部付属病院での運用開始を目指している。
ヒト血液から分離した顆粒球のラマンスペクトルを多数測定して,エノシノフィルペルオキシダーゼのラマンバンドの有無によって,好酸球を判別できることを確かめることができた。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

医療ラマンに特化した装置開発を行うことができた。自動測定ソフトウェアのインストール後は,ラマン分光学の専門家でなくても測定可能な体制を整えることができる。ヒト血液から分離した白血球の中から,好酸球を判別可能であることも明らかにすることができた。内視鏡に組み込み可能なファイバラマン装置を開発するための基礎的データを集積するための環境はほとんど整えることができたので,今後は内視鏡に組み込み可能なファイアバラマン装置開発を開始したいと考えている。

Strategy for Future Research Activity

内視鏡に組み込み可能なファイアバラマン装置を開発するにあたって,以下のような問題を解決する必要がある。1)好酸球の有無を判別可能な良好なラマンスペクトルの短時間測定,2)良好なラマンスペクトル測定の再現性の確保,3)測定したラマンスペクトルの自動解析
上記の問題のうち,1)と2)はハードウェア開発で,3)はソフトウェア開発である。ハードウェア開発に関しては,協力企業の分光科学研究所との共同研究によって進める予定である。ソフトウェアに関しては,近年発展が著しい,機械学習法の考えを導入してビッグデータから,エオシノフィルペルオキシダーゼを自動抽出可能なソフトウェアの開発を考えている。

  • Research Products

    (38 results)

All 2017 2016 Other

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 11 results,  Acknowledgement Compliant: 11 results) Presentation (26 results) (of which Int'l Joint Research: 23 results,  Invited: 9 results) Remarks (1 results)

  • [Journal Article] Biological and Medical Applications of Multivariate Curve Resolution Assisted Raman Spectroscopy2017

    • Author(s)
      Hemanth Noothalapati, Keita Iwasaki and Tatsuyuki Yamamoto
    • Journal Title

      Analytical Sciences

      Volume: 33 Pages: 15-22

    • DOI

      10.2116/analsci.33.15

    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Similarities and differences among eosinophilic esophagitis, proton-pump inhibitor-responsive esophageal eosinophilia, and reflux esophagitis: comparisons of clinical, endoscopic, and histopathological findings in Japanese patients2017

    • Author(s)
      Jiao D, Ishimura N, Maruyama R, Ishikawa N, Nagase M, Oshima N, Aimi M, Okimoto E, Mikami H, Izumi D, Okada M, Ishihara S, Kinoshita Y.
    • Journal Title

      Journal of Gastroenterology

      Volume: 52 Pages: 203-210

    • DOI

      10.1007/s00535-016-1213-1

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diagnosis and treatment of eosinophilic esophagitis in Japan2017

    • Author(s)
      Kinoshita Y, Ishimura N, Mishiro T, Ishihara S, Adachi K
    • Journal Title

      Esophagus

      Volume: 14 Pages: 66-75

    • DOI

      10.1007/s10388-016-0550-8

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Specific locations of linear furrows in patients with esophageal eosinophilia2017

    • Author(s)
      Okimoto E, Ishimura N, Okada M, Izumi D, Mikami H, Aimi M, Tanimura T, Mishiro T, Oshima N, Ishikawa N, Ishihara S, Adachi K, Maruyama R, Kinoshita Y
    • Journal Title

      Digestive Endoscopy

      Volume: 29 Pages: 49-56

    • DOI

      10.1111/den.12706

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Eosinophilic esophagitis versus proton pump inhibitor-responsive esophageal eosinophilia: Transcriptome analysis2017

    • Author(s)
      Shoda T, Matsuda A, Nomura I, Okada N, Orihara K, Mikami H, Ishimura N, Ishihara S, Matsumoto K, Kinoshita Y
    • Journal Title

      Journal of Allergy and Clinical Immunology

      Volume: - Pages: -

    • DOI

      10.1016/j.jaci.2016.11.028

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Label-free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis2016

    • Author(s)
      Hemanth Noothalapati, Takahiro Sasaki, Tomohiro Kaino, Makoto Kawamukai, Masahiro Ando, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 27789

    • DOI

      10.1038/srep27789

    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Blood identification and discrimination of human and nonhuman using portable Raman spectroscopy2016

    • Author(s)
      Junko Fujihara, Yasuhisa Fujita, Tatsuyuki Yamamoto, Naoki Nishimoto, Kaori Kimura-Kataoka, Satsuki Kurata, Yoshikazu Takinami, Toshihiro Yasuda, Haruo Takeshita
    • Journal Title

      International Journal of Legal Medicine

      Volume: - Pages: -

    • DOI

      10.1007/s00414-016-1396-2

    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Recent progress in research of eosinophilic esophagitis and gastroenteritis: review2016

    • Author(s)
      Kinoshita Y, Ishimura N, Oshima N, Mikami H, Okimoto E, Jiao DJ, Ishihara S.
    • Journal Title

      Digestion

      Volume: 93 Pages: 7-12

    • DOI

      10.1159/000441668

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Suitable biopsy site for detection of esophageal eosinophilia in eosinophilic esophagitis suspected cases2016

    • Author(s)
      Adachi K, Mishiro T, Tanaka S, Kinoshita Y.
    • Journal Title

      Digestive Endoscopy

      Volume: 28 Pages: 139-144

    • DOI

      10.1111/den.12555

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Eosinophilic Esophagitis, Eosinophilic Gastroenteritis, and Eosinophilic Colitis: Common Mechanisms and Differences between East and West2016

    • Author(s)
      Ishihara S, Kinoshita Y, Schoepfer A.
    • Journal Title

      Inflammatory Intestinal Diseases

      Volume: 1 Pages: 63-69

    • DOI

      10.1159/000445131

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sustained acid suppression by potassium-competitive acid blocker(P-CAB) may be an attractive treatment candidate for patients with eosionophilic esophagitis2016

    • Author(s)
      Ishimura N, Ishihara S, Kinoshita Y.
    • Journal Title

      American Journal of gastroenterology

      Volume: 111 Pages: 1203-1204

    • DOI

      10.1038/ajg.2016.167

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] In situ Raman spectral measurements of fresh blood of dry wood termite2016

    • Author(s)
      Tatsuyuki Yamamoto, Keita Iwasaki, Hemanth Noothalapati and Masaaki Azuma
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research / Invited
  • [Presentation] Visualizing pharmacokinetics and pharmacodynamics at the single cell level by Confocal Raman Microspectroscopy2016

    • Author(s)
      Hemanth Noothalapati, Venkatesh Kaliaperumal, Tatsuyuki Yamamoto and Hiro-o Hamaguchi
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] A time lapse study on the production efficiency of Paramylum and Wax ester in Euglena cells by Raman microspectroscopy2016

    • Author(s)
      Asuka Kaneko, Ayano Toyama, Suguru Uemura, Keita Iwasaki, Hemanth Noothalapati, Yuji Tanaka, Takahiro Ishikawa and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] Studies on a β-cyclodextrin derivative induced destruction process of liposomal membrane by confocal Raman microspectroscopy2016

    • Author(s)
      Chikako Yoshimoto, Akiko Miyazako, Keita Iwaski, Hemanth Noothalapati, Atsuhi Miyagawa, Hatsuo Yamamura, Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] An exploratory study on dancing body-like granule of symbiotic yeasts found in stag beetles by Raman microspectroscopy2016

    • Author(s)
      Hirokazau Kobayashi, Masahiko Tanahashi, Masahiro Ando, Hemanth Noothalapati, Takema Fukatsu, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] A resonance Raman spectroscopic study on the redox states of cytochrome b and c of ataxia and male sterility mouse2016

    • Author(s)
      Keita Iwasaki, Hemanth Noothalapati, Sheikh Abdullah, Atsushi Nagai and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] Elucidation of PV membrane differentiation mechanism in the green Paramecium and Chlorella symbiosis by Raman microspectroscopy2016

    • Author(s)
      Naoyuki Yasumura, Suguru Uemura, Keita Iwasaki, Hemanth Noothalapati, Yuuki Kodama and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] In vivo chemical imaging of sporulation process in fission yeast cells by confocal Raman microscopy2016

    • Author(s)
      Seiya Enokidzu, Keita Iwasaki, Suguru Uemura, Hemanth Noothalapati, Tomohiro Kaino, Makoto Kawamukai and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] Development of a non-bioptic diagnostic method for eosinophilic esophagitis using Raman spectroscopy2016

    • Author(s)
      Suguru Uemura, Hemanth Noothalapati, Naoki Ohshima, Masahiro Ando, Hiro-o Hamaguchi, Yoshikazu Kinoshita and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] Discrimination of breast cancer cells from normal cells by Raman spectroscopy and chemometrics2016

    • Author(s)
      Yui Suzuki, Keita Iwasaki, Hemanth Noothalapati, Riruke Maruyama and Tatsuyuki Yamamoto
    • Organizer
      Japan Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(淡路)
    • Year and Date
      2016-12-04 – 2016-12-07
    • Int'l Joint Research
  • [Presentation] Recent Advances in Biomedical Applications of Raman Spectroscopy2016

    • Author(s)
      Tatsuyuki Yamamoto, and Hemanth Noothalapati
    • Organizer
      10th International Symposium on Nanomedicine (ISNM2016)
    • Place of Presentation
      産業技術総合研究所(筑波)
    • Year and Date
      2016-11-24 – 2016-11-26
    • Int'l Joint Research / Invited
  • [Presentation] Detection of Eosinophils for the development of a new Raman diagnostic technique for Eosinophilic Esophagitis2016

    • Author(s)
      Tatsuyuki Yamamoto, Suguru Uemura, Hemanth Noothalapati, Naoki Oshima, Yoshikazu Kinoshita, Masahiro Ando, Hiro-o Hamaguchi
    • Organizer
      The 6th Biomedilca Molecular Imaging
    • Place of Presentation
      宜蘭(台湾)
    • Year and Date
      2016-11-12 – 2016-11-13
    • Int'l Joint Research / Invited
  • [Presentation] Application of Raman Spectroscopy on Living Cells and Tissues - Recent Works in Raman Project Center for Medical and Biological Applications of Shimane University2016

    • Author(s)
      Tatsuyuki Yamamoto and Hemanth Noothalapati
    • Organizer
      International Conference on Perspectives in Vibrational Spectroscopy
    • Place of Presentation
      ナクナウ(インド)
    • Year and Date
      2016-11-05 – 2016-11-08
    • Int'l Joint Research / Invited
  • [Presentation] クワガタムシに共生する酵母に見つかった未知のヘム酵素のラマン分光法による研究2016

    • Author(s)
      小林 洋和,岩崎 啓太,ヘマンス・ヌータラパティ,黄 祐敏,安藤 正浩,濵口 宏夫,棚橋 薫彦,深津 武馬,山本 達之
    • Organizer
      第34回Yeast Workshop
    • Place of Presentation
      島根大学(松江)
    • Year and Date
      2016-11-04 – 2016-11-05
  • [Presentation] Similarities and differences among eosinophilic esophagitis, proton pump inhibitor-responsive esophageal eosinophilia, and reflux esophagitis: comparisons of clinical, endoscopic, and histopathological findings in Japanese patients2016

    • Author(s)
      Jiao DJ, Ishimura N, Okada M, Mikami H, Izumi D, Okimoto E, Oshima N, Ishihara S, Kinoshita Y
    • Organizer
      Asian Pacific Digestive Week 2016
    • Place of Presentation
      神戸コンベンションセンター(神戸)
    • Year and Date
      2016-11-02 – 2016-11-05
    • Int'l Joint Research
  • [Presentation] Sustained acid suppression by potassium-competitive acid blocker (P-CAB) -possible treatment candidate for patients with eosinophilic esophagitis2016

    • Author(s)
      Ishimura N, Okada M, mikami H, Izumi D, Okimoto E, Mishiro T, Oshima N, Ishihara S, Kinoshita Y
    • Organizer
      Asian Pacific Digestive Week 2016
    • Place of Presentation
      神戸コンベンションセンター(神戸)
    • Year and Date
      2016-11-02 – 2016-11-05
    • Int'l Joint Research
  • [Presentation] Epidermaldifferentiation complex protein involucrin is down-regukated in eosinophikic esophagitis2016

    • Author(s)
      Oshima N, Ishihara S, Fukuba N, Kawashima K, Ishimura N, Kinoshita Y
    • Organizer
      Asian Pacific Digestive Week 2016
    • Place of Presentation
      神戸コンベンションセンター(神戸)
    • Year and Date
      2016-11-02 – 2016-11-05
    • Int'l Joint Research
  • [Presentation] Stable isotope probing coupled Raman microscopy: An efficient way to study single cell biochemistry2016

    • Author(s)
      Hemanth Noothalapati and Tatsuyuki Yamamoto
    • Organizer
      International Conference and Summit in Industrial and Pharmaceutical microbiology
    • Place of Presentation
      クアラルンプール(マレーシア)
    • Year and Date
      2016-10-17 – 2016-10-18
    • Int'l Joint Research / Invited
  • [Presentation] Imaging yeast cell wall architecture and studying the effect of anti-fungal drug terbinafine at the single cell level by Raman microscopy2016

    • Author(s)
      Hemanth Noothalapati and Tatsuyuki Yamamoto, International Conference and Summit in Industrial and Pharmaceutical microbiology
    • Organizer
      International Conference and Summit in Industrial and Pharmaceutical microbiology
    • Place of Presentation
      クアラルンプール(マレーシア)
    • Year and Date
      2016-10-17 – 2016-10-18
    • Int'l Joint Research
  • [Presentation] Recent Advances of Raman Project Center for Medical and Biological Applications, Shimane University2016

    • Author(s)
      Tatsuyuki Yamamoto
    • Organizer
      International Conference on Recent Trends in Engineering and Technology 2016(ICoRTET2016)
    • Place of Presentation
      コチ(インド)
    • Year and Date
      2016-10-03 – 2016-10-05
    • Int'l Joint Research / Invited
  • [Presentation] グアジニノ修飾シクロデキストリンの膜透過性能と細胞内局在2016

    • Author(s)
      久武 怜市,山口 純平,吉清 恵介,山本 達之
    • Organizer
      第33回シクロデキストリンシンポジウム
    • Place of Presentation
      香川大学(香川)
    • Year and Date
      2016-09-08 – 2016-09-09
  • [Presentation] Detection of Eosinophils on the Tissue of Esophagus by a Newly Developed Fiber Raman Spectrophotometer2016

    • Author(s)
      T. Yamamoto, S. Uemura, H. Noothalapati, N. Oshima, Y. Kinoshita, M. Ando, H. Hamaguchi
    • Organizer
      Fourth Taiwan International Symposium on Raman Spectroscopy
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2016-06-28 – 2016-07-01
    • Int'l Joint Research / Invited
  • [Presentation] A resonance Raman spectroscopic study on the redox states of cytochrome b and c in mitochondria isolated from mouse nerve cells with mitochondrial diseases2016

    • Author(s)
      K. Iwasaki, H. Noothalapati, S. Abdullah, A. Nagai, and T. Yamamoto
    • Organizer
      Fourth Taiwan International Symposium on Raman Spectroscopy
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2016-06-28 – 2016-07-01
    • Int'l Joint Research
  • [Presentation] A Raman spectroscopic study on the symbiotic yeast cells found in the mycangium of stag beetle2016

    • Author(s)
      H. Kobayashi, M. Tanahashi, M. Ando, H. Noothalapati, T. Fukatsu, H. Hamaguchi, and T. Yamamoto
    • Organizer
      Fourth Taiwan International Symposium on Raman Spectroscopy
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2016-06-28 – 2016-07-01
    • Int'l Joint Research
  • [Presentation] Medical Applications of Zinc Oxide Films and Nanoparticles2016

    • Author(s)
      Yasuhisa Fujita, Jie Lin, Ryousuke Tanino, Tatsuyuki. Yamamoto, Junko Fujihara, Haruo Takeshita and Takeshi Isobe
    • Organizer
      The 4th China-Japan Symposium on Nanomedicine
    • Place of Presentation
      北九州国際会議場(北九州)
    • Year and Date
      2016-05-11 – 2016-05-13
    • Int'l Joint Research / Invited
  • [Presentation] 島根大学 医・生物ラマンプロジェクトセンターの医療ラマンの取り組み2016

    • Author(s)
      山本 達之
    • Organizer
      ラマンバイオセンシングコンソーシアム
    • Place of Presentation
      関西学院大学 東京丸の内キャンパス(東京)
    • Year and Date
      2016-05-10 – 2016-05-10
    • Invited
  • [Remarks] 島根大学 医・生物ラマンプロジェクトセンター

    • URL

      http://fvraman.wixsite.com/raman

URL: 

Published: 2018-01-16  

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