Neuropathological analyses of cerebrovascular diseases using the brain ischemia model neurons: Development of novel forensic brain function biomarker

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04797
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: University of Yamanashi
• Principal Investigator: SHOJO Hideki 山梨大学, 大学院総合研究部, 准教授 (60284626)
• Co-Investigator(Kenkyū-buntansha): 馬淵 正 山梨大学, 総合研究部, 助教 (80150308)
• Co-Investigator(Renkei-kenkyūsha): ADACHI Noboru 山梨大学, 大学院総合研究部, 教授 (60282125)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳虚血 / ミトコンドリア形態 / ミトコンドリア機能 / 生活反応

Outline of Final Research Achievements

The goal of current study was to elucidate the mitochondrial vital response with brain ischemia, and to develop a novel forensic brain function biomarker using its vital response. Here, we examined the relationship between mitochondrial function and morphology using the brain ischemia model neurons. Under ischemic conditions, cell respiratory and mitochondrial activities significantly decreased in comparison with normoxic conditions. With their decrease in cellular functions, mitochondria were altered to simple filamentous and granular patterns from its complex fibrous structures. In addition, mitochondrial morphological changes were also retained in fixative specimens as a vital reaction. These results indicated that mitochondrial morphology would be a useful forensic biomarker of brain ischemia.

Free Research Field

法医学