FAPの次世代型複合的免疫療法によるアミロイド掃討作戦

2017 Fiscal Year Annual Research Report

• Project/Area Number: 15H04841
• Research Institution: Kumamoto University
• Principal Investigator: 安東 由喜雄 熊本大学, 大学院生命科学研究部(医), 教授 (20253742)
• Co-Investigator(Kenkyū-buntansha): 池田 徳典 熊本大学, 医学部附属病院, 病院教員 (00613530) ; 植田 光晴 熊本大学, 医学部附属病院, 講師 (60452885) ; 三隅 洋平 熊本大学, 大学院生命科学研究部(医), 助教 (80625781) ; 大林 光念 熊本大学, 大学院生命科学研究部(保), 教授 (90361899) ; 山下 太郎 熊本大学, 医学部附属病院, 特任教授 (90381003)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 神経科学 / 家族性アミロイドニューロパチー / トランスサイレチン / 抗体治療 / 免疫療法 / iPS細胞 / 神経分子病態学

Outline of Annual Research Achievements

家族性アミロイドポリニューロパチーは肝移植治療に加え、いくつかのオプションが出てきたが、組織に沈着したアミロイドを溶解する治療法はなく、これを開発することが根治治療の道を切り開くものと確信する。これまでのprothenaおよび化血研との共同研究で、TTR115-124およびTTR88-96のmonoclonal 抗体がin vitroのTTRアミロイドの形成を有効に抑制することがわかってきた。今後はさらにこの研究を発展させ、in vivoの研究、さらには臨床研究に発展させる。
1. FAP患者組織から抽出・分離・精製した抗原：FAP患者の剖検凍結組織をホモジネートしPras法でアミロイド線維を抽出した。この過程で得られる可溶性の分画もストックし、HPLC法で分取後にTTRオリゴマーの検出した。得られた各種のTTR形態を精製後に抗原として用いた。
解析の結果、TTR115-124はTTRがアミロイドになる折に確かにcryptic epitopeになることが確認されたが、加えて、TTR88-96も同様の抗原になることが新たに判明した。
２. in vitroの研究
TTR81-127のフラグメントがin vitroでアミロイドを形成することが判明したので、これを用い細胞レベルの実験を行った。細胞表面上でTTR81-127は、アミロイドを形成したが、TTR115-124およびTTR81-127抗体はこのアミロイドを有効に抑制した。また、iPS細胞をマクロファージ化しTTR89-96抗体およびTTR115-124 抗体を添加し、アミロイド抑制効果を見たところ、顕著な抑制効果が得られた。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

TR115-124はTTRがアミロイドになる折に確かにcryptic epitopeになることが確認されたが、加えて、化血研との共同研究に加えて、アメリカのベンチャー企業であるProthena社とも共同研究をしており、今年度予定しているin vivoの研究を行いながら、FAP患者に対する応用を目指して、確実に前に進んでいる。前臨床試験に入る具体的な段階に入っている。
現在までのところほぼ予定通りに研究は進展しており、あらたな研究の変更はない。

Strategy for Future Research Activity

平成30年度も前年度の研究を継続して実施し不足分を補う。In vivoの研究を完成させるとともに、モデル動物に対する副作用が増強されないか特に注意して解析する。また細胞療法の開発の研究も開始する。具体的には以下の通りである。
1. トランスジェニックラットへの抗体の投与：トランスジェニックラットにTTR89-96抗体およびTTR115-124 抗体を投与し、TTR沈着への効果を最終的に確認する。
2. マクロファージへ分化させ、各抗原の貪食効果を解析：健常人由来とFAP患者由来のiPS細胞からマクロファージへ分化させる。双方のiPS-MΦに（A）で得られた標的抗原を投与し、貪食能や反応性（サイトカイン産生）などに違いがあるか検証する。
3. モデル動物での効果検討：健常人由来とFAP患者由来のiPS-MΦを（A）で得られた標的抗原と共にインキュベーションした後にモデルラットへ投与し、TTR沈着量の変化などを検証する。
4. 副作用の確認：iPS-MΦの投与に伴う、副作用（生体での炎症反応）の確認をラットやウサギを用いて実施する。
以上の結果を経てFAP患者への臨床実験に速やかに移行する努力をする。

Research Products

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  • Author(s): Hayashi Y, Higashi T, Motoyama K, Jono H, Ando Y, Arima H
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• [Journal Article] A novel transthyretin variant V28S (p.V48S) with a double-nucleotide substitution in the same codon. (2018)
  • Author(s): Okada M, Misumi Y, Ueda M, Yamashita T, Masuda T, Tasaki M, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 231-232
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• [Journal Article] Diagnostic utility of cardiac troponin T level in patients with cardiac amyloidosis. (2018)
  • Author(s): Takashio S, Yamamuro M, Izumiya Y, Hirakawa K, Marume K, Yamamoto M, Ueda M, Yamashita T, Ishibashi-Ueda H, Yasuda S, Ogawa H, Ando Y, Anzai T, Tsujita K
  • Journal Title: ESC Heart Fail Volume: 5 Pages: 27-35
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• [Journal Article] Nationwide survey of 741 patients with systemic amyloid light-chain amyloidosis in Japan. (2018)
  • Author(s): Shimazaki C, Hata H, Iida S, Ueda M, Katoh N, Sekishima Y, Ikeda S, Yazaki M, Fukushima W, Ando Y
  • Journal Title: Intern Med Volume: 57 Pages: 181-187
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• [Journal Article] Genetic and clinical characteristics of hereditary transthyretin amyloidosis in endemic and non-endemic areas: experience from a single-referral center in Japan. (2018)
  • Author(s): Yamashita T, Ueda M, Misumi Y, Masuda T, Nomura T, Tasaki M, Takamatsu K, Sasada K, Obayashi K, Matsui H, Ando Y
  • Journal Title: J Neurol Volume: 265 Pages: 134-140
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• [Journal Article] First nationwide survey on systemic wild-type ATTR amyloidosis in Japan. (2018)
  • Author(s): Sekijima Y, Yazaki M, Ueda M, Koike H, Yamada M, Ando Y
  • Journal Title: Amyloid Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1080/13506129.2017.1409706.
  • Peer Reviewed
• [Journal Article] Late iodine enhancement and myocardial extracellular volume quantification in cardiac amyloidosis by using dual-energy cardiac computed tomography performed on a dual-layer spectral detector scanner. (2018)
  • Author(s): Oda S, Nakaura T, Utsunomiya D, Hirakawa K, Takashio S, Izumiya Y, Tsujita K, Hata H, Ando Y, Yamashita Y
  • Journal Title: Amyloid Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1080/13506129.2018.1424625
  • Peer Reviewed
• [Journal Article] Unwanted road to anaemia in transthyretin familial amyloid polyneuropathy may continue irrespective of tafamidis treatment. (2018)
  • Author(s): Ikeda T, Masuda T, Ueda M, Yamashita T, Misumi Y, Shinriki S, Ando Y
  • Journal Title: Ann Clin Biochem Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1177/0004563218754587.
  • Peer Reviewed
• [Journal Article] Diagnosis and management of transthyretin familial amyloid polyneuropathy in Japan: red-flag symptom clusters and treatment algorithm. (2018)
  • Author(s): Sekijima Y, Ueda M, Koike H, Misawa S, Ishii T, Ando Y
  • Journal Title: Orphanet J Rare Dis Volume: 13 Pages: 6
  • DOI: 10.1186/s13023-017-0726-x.
  • Peer Reviewed / Open Access
• [Journal Article] 家族性アミロイドポリニューロパチー. (2018)
  • Author(s): 安東 由喜雄
  • Journal Title: 今日の治療指針 Volume: 60 Pages: 960-961
• [Journal Article] Light chain deposition disease diagnosed with laser micro-dissection, liquid chromatography, and tandem mass spectrometry of nodular glomerular lesions. (2017)
  • Author(s): Kasagi T, Nobata H, Suzuki K, Miura N, Banno S, Takami A, Yamashita T, Ando Y, Imai H
  • Journal Title: Intern Med Volume: 56 Pages: 61-66
  • DOI: 10.2169/internalmedicine.56.7275
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• [Journal Article] Clinicopathological and biochemical findings of thyroid amyloid in hereditary transthyretin amyloidosis with and without liver transplantation. (2017)
  • Author(s): Huang G, Ueda M, Tasaki M, Yamashita T, Misumi Y, Masuda T, Suenaga G, Inoue Y, Kinoshita Y, Matsumoto S, Mizukami M, Tsuda Y, Nomura T, Obayashi K, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 24-29
  • DOI: 10.1080/13506129.2016.1276440
  • Peer Reviewed
• [Journal Article] Identification and assessment of cardiac amyloidosis by myocardial strain analysis of cardiac magnetic resonance imaging. (2017)
  • Author(s): Oda S, Utsunomiya D, Nakaura T, Yuki H, Kidoh M, Morita K, Takashio S, Yamamuro M, Izumiya Y, Hirakawa K, Ishida T, Tsujita K, Ueda M, Yamashita T, Ando Y, Hata H, Yamashita Y
  • Journal Title: Circ J Volume: 81 Pages: 1014-1021
  • DOI: 10.1253/circj.CJ-16-1259
  • Peer Reviewed / Open Access
• [Journal Article] Novel screening for transthyretin amyloidosis by using fat ultrasonography. (2017)
  • Author(s): Misumi Y, Ueda M, Yamashita T, Masuda T, Kinoshita Y, Tasaki M, Nagase T, Ando Y
  • Journal Title: Ann Neurol Volume: 81 Pages: 604-608
  • DOI: 10.1002/ana.24914
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• [Journal Article] A multicenter cross sectional study for bone-articular lesions associated with dialysis related amyloidosis in Japan. (2017)
  • Author(s): Nishi S, Hoshino J, Yamamoto S, Goto S, Fuji H, Ubara Y, Motomiya Y, Morita H, Takaichi K, Yamagata K, Shigematsu T, Ueda M, Ando Y
  • Journal Title: Nephrology Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1111/nep.13077
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• [Journal Article] Early skin denervation in hereditary and iatrogenic transthyretin amyloid neuropathy. (2017)
  • Author(s): Masuda T, Ueda M, Suenaga G, Misumi Y, Tasaki M, Izaki A, Yanagisawa Y, Inoue Y, Motokawa H, Matsumoto S, Mizukami M, Arimura A, Deguchi T, Nishio Y, Yamashita T, Inomata Y, Obayashi K, Ando Y
  • Journal Title: Neurology Volume: 88 Pages: 2192-2197
  • DOI: 10.1212/WNL.0000000000004016
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• [Journal Article] Inflammatory state exists in familial amyloid polyneuropathy that may be triggered by mutated transthyretin. (2017)
  • Author(s): Suenaga G, Ikeda T, Masuda T, Motokawa H, Yamashita T, Takamatsu K, Misumi Y, Ueda M, Matsui H, Senju S, Ando Y
  • Journal Title: Sci Rep Volume: 7 Pages: 1579
  • DOI: 10.1038/s41598-017-01775-4
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• [Journal Article] Sushi repeat-containing protein 1: a novel disease-associated molecule in cerebral amyloid angiopathy. (2017)
  • Author(s): Inoue Y, Ueda M, Tasaki M, Takeshima A, Nagatoshi A, Masuda T, Misumi Y, Kosaka T, Nomura T, Mizukami M, Matsumoto S, Yamashita T, Takahashi H, Kakita A, Ando Y
  • Journal Title: Acta Neuropathol Volume: 134 Pages: 605-617
  • DOI: 10.1007/s00401-017-1720-z.
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• [Journal Article] Cardiovascular magnetic resonance myocardial T1 mapping to detect and quantify cardiac involvement in familial amyloid polyneuropathy. (2017)
  • Author(s): Oda S, Utsunomiya D, Morita K, Nakaura T, Yuki H, Kidoh M, Hirata K, Taguchi N, Tsuda N, Shiraishi S, Namimoto T, Hirakawa K, Takashio S, Izumiya Y, Yamamuro M, Hokimoto S, Tsujita K, Ueda M, Yamashita T, Ando Y, Yamashita Y
  • Journal Title: Eur Radiol Volume: 27(11) Pages: 4631-4638
  • DOI: 10.1007/s00330-017-4845-5
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• [Journal Article] A late-onset case of hereditary transthyretin amyloidosis with a novel compound heterozygous mutation. (2017)
  • Author(s): Matsumoto S, Ueda M, Yamashita T, Amano T, Misumi Y, Tasaki M, Masuda T, Mizukami M, Furuya H, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 97-98
  • DOI: 10.1080/13506129.2017.1286585.
  • Peer Reviewed
• [Journal Article] Identification of amyloid precursor protein from autopsy and biopsy specimens using LMD-LC-MS/MS: the experience at Kumamoto University. (2017)
  • Author(s): Tasaki M, Ueda M, Obayashi K, Kinoshita Y, Matsumoto S, Mizukami M, Masuda T, Misumi Y, Yamashita T, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 167-168
  • DOI: 10.1080/13506129.2017.1286583.
  • Peer Reviewed
• [Journal Article] In vitro and in vivo assessment of novel derivatives for detecting various amyloid deposits. (2017)
  • Author(s): Izaki A, Obayashi K, Suenaga G, Ueda M, Yamashita T, Tasaki M, Yanagisawa Y, Masuda T, Misumi Y, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 17
  • DOI: 10.1080/13506129.2017.1287070.
  • Peer Reviewed
• [Journal Article] A case with a novel variant transthyretin A36D presenting cardiac phenotype. (2017)
  • Author(s): Nomura T, Yamashita T, Misumi Y, Ueda M, Masuda T, Tasaki M, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 100
  • DOI: 10.1080/13506129.2017.1287072.
  • Peer Reviewed
• [Journal Article] Iatrogenic systemic transthyretin amyloid deposits in a case with domino liver transplantation: an autopsy case study. (2017)
  • Author(s): Tsuda Y, Misumi Y, Ueda M, Tasaki M, Huang G, Masuda T, Suenaga G, Kinoshita Y, Obayashi K, Yamashita T, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 125
  • DOI: 10.1080/13506129.2017.1287071.
  • Peer Reviewed
• [Journal Article] Antibody therapy for transthyretin-related hereditary amyloid polyneuropathy: another therapeutic option. (2017)
  • Author(s): Ando Y, Ueda M
  • Journal Title: Amyloid Volume: 24 Pages: 113-114
  • DOI: 10.1080/13506129.2017.1293514
  • Peer Reviewed
• [Journal Article] Long-term effects of liver transplantation on small-fiber dysfunction in Japanese transthyretin (ATTR) V30M hereditary amyloidosis with polyneuropathy (FAP). (2017)
  • Author(s): Obayashi K, Ueda M, Yamashita T, Tasaki M, Izaki A, Yanagisawa Y, Masuda T, Misumi Y, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 99
  • DOI: 10.1080/13506129.2017.1286586.
  • Peer Reviewed
• [Journal Article] Hereditary transthyretin amyloidosis associated with a transthyretin variant Thr59Arg. (2017)
  • Author(s): Watanabe T, Obayashi K, Misumi Y, Tasaki M, Shinriki S, Ando T, Akagami T, Ueda M, Yamashita T, Hirotani S, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 119
  • DOI: 10.1080/13506129.2017.1294057.
  • Peer Reviewed
• [Journal Article] Disruption of blood-nerve barriers in hereditary transthyretin (ATTR) amyloidosis. (2017)
  • Author(s): Koike H, Ikeda S, Takahashi M, Kawagashira Y, Iijima M, Misumi Y, Ando Y, Ikeda SI, Katsuno M, Sobue G
  • Journal Title: Amyloid Volume: 24 Pages: 89-90
  • DOI: 10.1080/13506129.2017.1278691.
  • Peer Reviewed
• [Journal Article] Clinicopathological and biochemical findings of late-onset hereditary transthyretin amyloidosis 16 years after liver transplantation: an autopsy case study. (2017)
  • Author(s): Mizukami M, Ueda M, Tasaki M, Misumi Y, Masuda T, Matsumoto S, Yamashita T, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 122
  • DOI: 10.1080/13506129.2017.1295941.
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of a patient with ureteral amyloidosis. (2017)
  • Author(s): Yanagisawa Y, Obayashi K, Tasaki M, Izaki A, Masuda T, Misumi Y, Ueda M, Yamashita T, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 101
  • DOI: 10.1080/13506129.2017.1287696.
  • Peer Reviewed
• [Journal Article] Establishment of a diagnostic center for amyloidosis in Japan by Kumamoto University. (2017)
  • Author(s): Yamashita T, Ueda M, Tasaki M, Masuda T, Misumi Y, Takamatsu K, Obayashi K, Ando Y
  • Journal Title: Amyloid Volume: 24 Pages: 169-170
  • DOI: 10.1080/13506129.2017.1295951.
  • Peer Reviewed
• [Journal Article] Utility of single-photon emission computed tomography/computed tomography fusion imaging with 99 mTc-Pyrophosphate scintigraphy in the assessment of cardiac transthyretin amyloidosis. (2017)
  • Author(s): Tsuda N, Shiraishi S, Oda S, Ogasawara K, Sakamoto F, Tomiguchi S, Fujisue K, Takashio S, Izumiya Y, Tsujita K, Ando Y, Yamashita Y
  • Journal Title: Circ J Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1253/circj.CJ-17-1111.
  • Peer Reviewed / Open Access
• [Journal Article] ALアミロイドーシスにおける質量分析を用いた沈着ペプチド解析. (2017)
  • Author(s): 杉本 俊哉, 田﨑 雅義，山下 太郎，植田 光晴，安東 由喜雄，大林 光念，奥野 豊，畑 裕之
  • Journal Title: 臨床血液 Volume: 58 Pages: 589-594
  • DOI: 10.11406/rinketsu.58.589
  • Peer Reviewed
• [Journal Article] 免疫系と自律神経系のインターフェース. (2017)
  • Author(s): 中根 俊成, 向野 晃弘, 安東 由喜雄
  • Journal Title: 日本臨床免疫学会会誌 Volume: 40 Pages: 352-360
  • DOI: 10.2177/jsci.40.352
  • Peer Reviewed / Open Access
• [Journal Article] 家族性アミロイドポリニューロパチーの臨床と治療. (2017)
  • Author(s): 安東 由喜雄
  • Journal Title: 最新医学 Volume: 72(8) Pages: 1193-1199
  • Peer Reviewed / Open Access
• [Journal Article] タファミジス：遺伝性トランスサイレチンアミロイドーシスに対する蛋白質安定化剤. (2017)
  • Author(s): 山下 太郎, 安東 由喜雄
  • Journal Title: 心臓（日本医学出版・日本循環器学会）Heart’s Selection特集 循環器領域における代謝性疾患に対する新薬 Volume: 49(5) Pages: 428-443
  • Peer Reviewed
• [Journal Article] トランスサイレチン（ATTR）型脳アミロイド血管症/軟膜アミロイドーシス. (2017)
  • Author(s): 山下 太郎, 安東 由喜雄
  • Journal Title: 日本神経治療学会 標準的神経治療 アミロイドーシス Volume: 印刷中 Pages: 印刷中
• [Journal Article] アミロイドーシスを疑う臨床症状と症候. (2017)
  • Author(s): 山下 太郎, 安東 由喜雄
  • Journal Title: 医学のあゆみ 医歯薬出版株式会社. 別冊 Volume: 印刷中 Pages: 33-40
  • Peer Reviewed
• [Journal Article] アミロイド蛋白質の免疫組織化学的・蛋白質化学的同定法. (2017)
  • Author(s): 植田光晴・安東由喜雄
  • Journal Title: 医学のあゆみ 医歯薬出版株式会社. 別冊 Volume: 印刷中 Pages: 41-45
  • Peer Reviewed
• [Journal Article] 遺伝性ATTR型脳アミロイド血管症／家族性ATTR型眼軟膜アミロイドーシスの臨床像. (2017)
  • Author(s): 山下 太郎, 安東 由喜雄
  • Journal Title: 神経内科 Volume: 88(3) Pages: 305-310
  • Peer Reviewed
• [Presentation] Correlation of heart rate variability analysis and MIBG myocardial scintigraphy in patients with PD (2018)
  • Author(s): Nakahara K, Nakane S, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Quantification of skin denervation in patients with transthyretin amyloid neuropathy (2018)
  • Author(s): Masuda T, Ueda M, Misumi Y, Matsumoto S, Nomura T, Tasaki M, Yamashita T, Sonoda Y, Obayashi K, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Pupillary autonomic dysfunction as an early bioker in patients with hereditary transthyretin amyloidosis (2018)
  • Author(s): Masuda T, Ueda M, Nagatoshi C, Misumi Y, Nomura T, Tasaki M, Yamashita T, Obayashi K, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Two siblings with hereditary transthyretin amyloidosis: a rare TTR variant T49S (pT69S) (2018)
  • Author(s): Masataka Y, Masuda T, Yamashita T, Ueda M, Misumi Y, Ando T, Masuyama T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis, , ,
  • Int'l Joint Research
• [Presentation] A transgenic Caenorhabditis elegans expressing transthyretin as a model in transthyretin amyloidosis (2018)
  • Author(s): Tsuda Y, Ueda M, Yamanaka K, Masuda T, Misumi Y, Ogura T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] DNA aptamer raised against the C-terminal domain of β2-microglobulin inhibits the fibrillogenesis of ⊿N6β2m (2018)
  • Author(s): Tsuda Y, Higashimoto Y, Fukasawa K, Motomiya Y, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Rapid detection of mutated transthyretin using direct MALDI-TOF MS. (2018)
  • Author(s): Nomura T, Ueda M, Matsumoto S, Mizukami M, Nakamura M, Kanenawa K, Tsuda Y, Inoue Y, Masuda T, Misumi Y, Yamashita T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Analyses of prostatic corpora amylacea (2018)
  • Author(s): Kanenawa K, Ueda M, Matsumoto S, Mizukami M, Isoguchi A, Yamamoto S, Tsuda Y, Misumi Y, Masuda T, Obayashi K, Yamashita T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Transthyretin Fragments in Peripheral Blood Cells (2018)
  • Author(s): Matsumoto S, Ueda M, Tasaki M, Misumi Y, Masuda T, Mizukami M, Kanenawa K, Nakamura M, Obayashi K, Yamashita T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Mechanism of the insulin resistance at the site of insulin amyloidoma (2018)
  • Author(s): Nakamura M, Misumi Y, Oka W, Nomura T, Mizukami M, Matsumoto S, Ueda M, Yamashita T, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Genetical and geographical features of hereditary transthyretin amyloidosis in (2018)
  • Author(s): Yamashita T, Ueda M, Misumi Y, Masuda T, Nomura T, Tasaki M, Takamatsu K, Sasada K, Obayashi K, Matsui H, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Is liver transplantation effective for non-V30M hereditary transthyretin amyloidosis ? The XVIth International Symposium on Amyloidosis (2018)
  • Author(s): Yamashita T, Ueda M, Nomura T, Tsuda Y, Inoue Y, Masuda T, Misumi Y, Takamatsu K, Obayashi K, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Tafamidis or transplantation of the liver: Which should be chosen for hereditary transthyretin amyloidosis? (2018)
  • Author(s): Yamashita T, Kato Y, Ueda M, Inoue Y, Masuda T, Misumi Y, Nomura T, Watari M, Obayashi K, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Transthyretin amyloid-related cerebral angitis after liver transplantation (2018)
  • Author(s): Yamashita T, Ohnishi K, Ueda M, Masuda T, Inoue Y, Misumi Y, Ueda A, Obayashi K, Takeya M, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Changes in nerve excitability indices in hereditary transthyretin amyloidosis (2018)
  • Author(s): Yamashita T, Ueda M, Misawa S, Inoue Y, Masuda T, Misumi Y, Takamatsu K, Obayashi K, Kuwabara S, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Patisiran, an investigational RNAi therapeutic for patients with hereditary transthyretin-mediated (hATTR) amyloidosis: Phase 3 APOLLO study subanalysis of ese patients (2018)
  • Author(s): Yamashita T, Sekijima Y, Koike H, Ueda M, Yoshinaga T, Kodaira M, Sobue G, Katsuno M, Singh T, Hashimoto Y, Kadam K, A Hou, Ando Y
  • Organizer: The XVIth International Symposium on Amyloidosis
  • Int'l Joint Research
• [Presentation] Evaluation of MIBG scintigraphy in the Movement Disorder Society Parkinson’s disease criteria (2017)
  • Author(s): Ikenoshita S, Ikeda T, Yamashita S, Sakamoto F, Shiraishi S, Nakahara K, Masuda T, Ando Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Memantine attenuates vascular amyloid β deposits and spontaneous hemorrhages in mouse models of cerebral amyloid angiopathy (2017)
  • Author(s): Inoue Y, Ueda M, Masuda T, Misumi Y, Yamashita T, Ando Y
  • Organizer: Alzheimer's Association International Conference
  • Int'l Joint Research
• [Presentation] Early skin denervation in transthyretin amyloid neuropathy. Driving change: Novel research into early screening and diagnosis of hereditary transthyretin amyloidosis polyneuropathy (2017)
  • Author(s): Ueda M, Masuda T, Ando Y
  • Organizer: PNS 2017 annual meeting
  • Int'l Joint Research
• [Presentation] Amyloid formation and toxicity of fragmented transthyretin (2017)
  • Author(s): Ueda M, Misumi Y, Masuda T, Tsuda Y, Inoue Y, Tasaki M, Yamashita T, Ando Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Novel findings of amyloidosis: Analyses by mass spectrometry. Clinical Mass Spectrometry Meeting Anatomical and Chemical Pathology (2017)
  • Author(s): Ueda M, Tasaki M, Inoue Y, Ando Y
  • Organizer: The 29th World Congress of World Association of Societies of Pathology and Laboratory Medicine
  • Int'l Joint Research
• [Presentation] Novel diagnostic and treatment strategies in amyloidosis (2017)
  • Author(s): Ueda M, Masuda T, Misumi Y, Tasaki M, Inoue Y, Yamashita T, Ando Y
  • Organizer: Medical and Nursing Symposium between Shandong University and Kumamoto University
• [Presentation] Skin denervation in patients with hereditary and wild-type transthyretin amyloidosis (2017)
  • Author(s): Masuda T, Ueda M, Misumi Y, Nomura T, Tasaki M, Yamashita T, Obayashi K, Ando Y
  • Organizer: International Society for Autonomic Neuroscience
  • Int'l Joint Research
• [Presentation] Impact of skin denervation in patients with wild-type transthyretin amyloidosis (2017)
  • Author(s): Masuda T, Ueda M, Misumi Y, Suenaga G, Matsumoto S, Nomura T, Tasaki M, Yamashita T, Obayashi K, Ando Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Pupillary autonomic nervous dysfunction in hereditary transthyretin amyloidosis (2017)
  • Author(s): Nagatoshi C, Masuda T, Ueda M, Misumi Y, Nomura T, Tasaki M, Yamashita T, Obayashi K, Ando Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Amyloid angiopathy in hereditary transthyretin amyloidosis (2017)
  • Author(s): Nomura T, Misumi Y, Ueda M, Masuda T, Yamashita T, Ando Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Transthyretin related amyloidosis -Diagnosis, pathogenesis, and therapies- (2017)
  • Author(s): Ando Y
  • Organizer: Pre-Conference Seminar of The 5th Hong Kong Neurological Congress and 30th annual Scientific Meeting ofThe Hong Kong Neurological Society
  • Int'l Joint Research / Invited
• [Presentation] 脳アミロイドアンギオパチーの新規病態関連因子SRPX1の解析． (2017)
  • Author(s): 井上泰輝，植田光晴，田﨑雅義，竹島明，永利聡仁，増田曜章，三隅洋平，小阪崇幸，野村隼也，水上真由美，松本紗也加，山下太郎，高橋均，柿田明美，安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 脳アミロイドアンギオパチーの新規病態関連因子SRPX1の解析． (2017)
  • Author(s): 井上泰輝，植田光晴，田﨑雅義，竹島明，永利聡仁，増田曜章，三隅洋平，小阪崇幸，野村隼也，水上真由美，松本紗也加，山下太郎，高橋均，柿田明美，安東由喜雄
  • Organizer: 第36回日本認知症学会学術集会
• [Presentation] 遺伝性トランスサイレチンアミロイドーシス患者からドミノ肝移植を受けた二次レシピエントに生じる医原性アミロイドーシスの臨床像の解析. (2017)
  • Author(s): 三隅洋平、植田光晴、増田曜章、野村隼也、山下太郎、安東由喜雄
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] 遺伝性トランスサイレチンアミロイドーシス早期診断のポイント. (2017)
  • Author(s): 三隅洋平、安東由喜雄
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] TTRアミロイドーシス実験モデルを用いた病態解析及び治療法の開発．シンポジウム「アミロイドーシス研究入門: 線維形成実験からマウスモデルまで」 (2017)
  • Author(s): 植田光晴、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] トランスサイレチンフラグメントのアミロイド形成および細胞毒性の解析． (2017)
  • Author(s): 植田光晴、三隅洋平、増田曜章、水口峰之、井上泰輝、津田幸元、野村隼也、松本沙也加、水上真由美、金縄京佑、中村真琴、山下太郎、安東由喜雄
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] 遺伝性脳アミロイドアンギオパチー. (2017)
  • Author(s): 植田明彦、山下太郎、安東由喜雄
  • Organizer: 第49回日本動脈硬化学会
• [Presentation] 生検皮膚組織を用いたATTRアミロイドーシスの病態評価. (2017)
  • Author(s): 増田曜章、植田光晴、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 野生型トランスサイレチンアミロイドーシスにおける小径線維障害の検討. (2017)
  • Author(s): 増田曜章、植田光晴、三隅洋平、野村隼也、松本紗也加、田崎雅義、小阪崇幸、山下太郎、大林光念、安東由喜雄
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] TTRアミロイト?沈着を来たす線虫モテ?ルの開発に向けた検討. (2017)
  • Author(s): 津田幸元、植田光晴、山中邦俊、小椋光、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 遺伝性ATTRアミロイドーシスにおける微小血管障害の解析. (2017)
  • Author(s): 野村隼也、三隅洋平、増田曜章、植田光晴、山下太郎、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 肝移植後に長期経過した遺伝性トランスサイレチンアミロイドーシスの解析 (2017)
  • Author(s): 野村隼也、植田光晴、大嶋俊範、三隅洋平、増田曜章、山下太郎、安東由喜雄
  • Organizer: 第35回日本神経治療学会総会
• [Presentation] トランスサイレチンアミロイドーシス線虫モデルを用いた治療薬の検討. (2017)
  • Author(s): 金縄 京佑、津田 幸元、植田 光晴、山中 邦俊、小椋 光、安東 由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 血球成分におけるトランスサイレチン断片化機構の解析. (2017)
  • Author(s): 松本紗也加、植田光晴、田崎雅義、三隅洋平、増田曜章、水上真由美、金縄京佑、中村真琴、大林光念、山下太郎、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 変異型TTR Tyr114Cys型遺伝性トランスサイレチンアミロイドーシス新規モデルマウスの作成および病理・生化学的解析. (2017)
  • Author(s): 水上真由美、植田光晴、三隅洋平、増田曜章、松本紗也加、大林光念、山下太郎、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Presentation] 線維芽細胞がTTRアミロイド沈着形態に与える影響に関する三次元培養系解析. (2017)
  • Author(s): 中村真琴、三隅洋平、木下祐美子、野村隼也、植田光晴、増田曜章、山下太郎、安東由喜雄
  • Organizer: 第5回日本アミロイドーシス研究会学術集会，京都
• [Book] Clinical Questions ＆ Pearls 「末梢神経疾患」 (2018)
  • Author(s): 増田 曜章, 安東 由喜雄
  • Total Pages: 404
  • Publisher: 中外医学社
  • ISBN: 978-4-498-22898-6
• [Book] 映画に描かれた疾患と募る想い (2018)
  • Author(s): 安東 由喜雄
  • Total Pages: 288
  • Publisher: 医歯薬出版
  • ISBN: 978-4-263-73180-2
• [Book] 最新 アミロイドーシスのすべて 診療ガイドライン2017とQ＆A (2017)
  • Author(s): 安東 由喜雄
  • Total Pages: 264
  • Publisher: 医歯薬出版
  • ISBN: 978-4-263-73175-8