Radiosensitization based on intracellular redox control in cancer stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04904
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: University of Toyama
• Principal Investigator: Kondo Takashi 富山大学, 大学院医学薬学研究部(医学), 名誉教授 (40143937)
• Co-Investigator(Kenkyū-buntansha): 田渕 圭章 富山大学, 研究推進機構 研究推進総合支援センター, 教授 (20322109) ; 松谷 裕二 富山大学, 大学院医学薬学研究部(薬学), 教授 (50255858) ; 小川 良平 富山大学, 大学院医学薬学研究部(医学), 准教授 (60334736) ; 趙 慶利 富山大学, 大学院医学薬学研究部(医学), 助教 (90313593)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: スルファサラジン / 幹細胞 / 放射線

Outline of Final Research Achievements

Sulfasalazine (SSZ) is an inhibitor of the cystine-glutamate antiporter known to reduce intracellular glutathione level and increase cellular oxidative stress, giving its anti-tumor potential. SSZ enhanced radiation-induced apoptosis in Molt-4 cells via the involvement of both intrinsic and extrinsic apoptotic pathways. In addition, to understand characteristics of stem cells, the immortalized human amniotic mesenchymal cells (iHAMs) and immortalized human amniotic epithelial cells (iHAEs) were utilized. To reveal response of these cells against X-rays and hydrogen peroxide, intracellular reactive oxygen species (ROS), cell viability, and apoptois were examined. Intracellular ROS level significantly increased in iHAMs after treatment, consequently cell viability also significantly decreased in iHAMs, but not in iHAEs. Radiation-induced apoptosis was also higher in iHAMs. Furthermore, proteins related to oxidative stress were also determined and detailed mechanism was discussed.

Free Research Field

放射線基礎医学