Development of transplantation therapy using hypoxically preconditioned cell sheets for lower limb ischemic ulcers

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04939
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Yamaguchi University
• Principal Investigator: HAMANO Kimikazu 山口大学, 大学院医学系研究科, 教授 (60263787)
• Co-Investigator(Kenkyū-buntansha): 細山 徹 山口大学, 大学院医学系研究科, 助教 (20638803) ; 上野 耕司 山口大学, 医学部附属病院, 助教 (30736070) ; 森景 則保 山口大学, 医学部附属病院, 講師 (50335741) ; 李 桃生 長崎大学, 原爆後障害医療研究所, 教授 (50379997)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞シート / 再生医療 / 難治性皮膚潰瘍

Outline of Final Research Achievements

In this study, cell-mixed sheets were consisting of peripheral blood mononuclear cells (PBMNCs) secreting growth factors and fibroblasts secreting collagens on cutaneous skin ulcers. Growth factors secreted from PBMNCs increased the production of VEGF protein in Fibroblasts. cell-mixed sheets transplanted into full-thickness skin defects promoted the wound healing compared to no treatment in mice model. Cell-mixed sheets transplanted into full-thickness skin were not detected in tissues after healing in syngeneic transplantation models. Our data suggested that cell-mixed sheets might be a new treatment method for cutaneous skin ulcers.

Free Research Field

再生医療、心臓血管外科学