Analysis of control method of bacterial pneumonia utilizing functional glycolipids and researching its molecular mechanism

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kyoto Pharmaceutical University (2016-2017); Niigata University (2015)
• Principal Investigator: ODA MASATAKA 京都薬科大学, 薬学部, 教授 (00412403)
• Co-Investigator(Kenkyū-buntansha): 土門 久哲 新潟大学, 医歯学系, 助教 (00594350) ; 寺尾 豊 新潟大学, 医歯学系, 教授 (50397717)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 機能性糖脂質 / 肺炎 / 細胞外捕獲網 / 肺炎球菌 / 緑膿菌

Outline of Final Research Achievements

In this research project, experiments were carried out to analyze the effect of newly developed water-soluble glycolipid (WSG) on bacterial pneumonia and its mechanism. As a result, the following three things were revealed.1) WSG induced the extracellular release of a net structure containing nucleic acid as a main component in macrophages or neutrophils. Especially in macrophages, it was found that mitochondria-derived nucleic acid was released with little cell death and trapped Streptococcus pneumoniae and Pseudomonas aeruginosa.2) Analysis using inhibitors and knockdown cells suggested that the WSG act on the mincle receptor other than TLR4 receptor.
3) Pneumonia and mortality caused by S. pneumoniae were suppressed by nasal administration of WSG.

Free Research Field

細菌学、免疫学、薬学、口腔細菌学