Multistep syntrophy in the degradation of amino acids and branched-chain fatty acids in anaerobic ecosystem

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05331
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Environmental dynamic analysis
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Narihiro Takashi 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20421844)
• Research Collaborator: YAMADA Masayoshi 鹿児島工業高等専門学校, 都市環境デザイン工学科, 准教授 (80469593) ; KURODA Kyohei 都城工業高等専門学校, 物質工学科, 助教 (50783213) ; NOBU Masaru 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (40805644) ; LIU Wen-Tso イリノイ大学, 土木環境工学系, 教授 ; LEE Po-Heng 香港理工大学, 土木環境工学系, 助教 ; TANG Yueqin 四川大学, 環境工学系, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 微生物ゲノム / 廃水処理 / メタゲノム / アミノ酸

Outline of Final Research Achievements

Metagenomic analysis of a methanogenic microbial community in an anaerobic bioreactor treating amino acid-containing wastewater revealed synergistic metabolic network of syntrophic substrate-oxidizing bacteria, methanogens, and functionally unknown microorganisms. A laboratory-scale upflow anaerobic sludge blanket reactor fed with wastewater discharged from soy sauce processing manufactory was operated at 20°C. We successfully recovered metagenomic bins of dominant microbes in the reactor. Metabolic reconstruction suggested that dominant microorganisms perform fermentative and syntrophic degradation of amino acids and catabolic by-products facilitated by various energy conservation systems. Thus, diverse anaerobic organisms may unite to form a metabolic network to perform complete degradation of amino acids in the methanogenic microbiota.

Free Research Field

環境微生物学