2017 Fiscal Year Final Research Report
Comprehensive creation of antibody-like ligands for human proteomics
| Project/Area Number |
15H05372
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | University of Yamanashi (2016-2017)
National Institute of Advanced Industrial Science and Technology (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | プロテオミクス / HUPEX / ヒト腕型ロボット / 無細胞タンパク質合成系 / 部位特異的ビオチン化 / cDNAクローン / PUREシステム / in vitro virus |
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| Outline of Final Research Achievements |
By integrating cyclic peptide ligand creation using PURE system and mRNA display with HUPEX to prepare immobilized human proteins from its encoding cDNA clones, we successfully created novel cyclic peptide ligands targeted to the human proteins in a full cell-free format. By combining the system with humanoid robot system, human protein-binding ligands created fully automatically were successfully applied to proteomic analysis.
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| Free Research Field |
ケミカルバイオロジー
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