Development of a Screening Method for Novel Biotribological Molecules using Surface Plasmon Resonance

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05506
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Design engineering/Machine functional elements/Tribology
• Research Institution: Doshisha University (2016-2017); Kyoto University (2015)
• Principal Investigator: YAMAMOTO KOJI 同志社大学, 生命医科学部, 准教授 (70536565)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 表面プラズモン共鳴 / 関節 / バイオトライボロジ― / ルブリシン

Outline of Final Research Achievements

To identify novel biotribological molecules for articular cartilage, a newly system which is able to detect both bio-chemical and physical interactions between them was developed using surface plasmon resonance (SPR). In this system, molecules involving in articular lubrication which can interact with lubricin or hyaluronic acid were initially identified using molecular biological methods, and then selected according to whether those molecules can affect mechanical interactions at the material interface. To detect unknown time-dependent interaction between molecules in real-time using SPR, the incident light had a certain distribution of incident angle, which could avoid a delay attributed to the mechanical scanning of the SPR angle. In addition, to concurrently detect the mechanical interaction between molecules on this SPR device, grafted molecule on the gold film using self-assembled monolayer was applied.

Free Research Field

機械工学