To develop the effective treatment for hepatic fibrosis using Adipose Tissue-Derived.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05679
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: National Institutes of Biomedical Innovation, Health and Nutrition
• Principal Investigator: OKURA Hanayuki 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 難治性疾患研究開発・支援センター, 研究調整専門員 (20589684)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脂肪組織由来多系統前駆細胞 / 細胞シート / 肝線維症 / 再生医療 / 再生医学 / 肝硬変 / ADMPC / 肝線維化

Outline of Final Research Achievements

As a result of examining cytokine secretion from ADMPC, cytokine having the curative properties of dissolving hepatic fibrosis was secreted in a greater amount than fibroblasts or adipose tissue-derived cells. This mechanism might be Mode-of-Action.
In order to investigate conditions for producing cell sheets, culturing ADMPCs with TGF-β were examined. The efficiency of producing cell sheets were good, but unfortunately the expression of anti-inflammatory cytokines decreased. It was found to be unfavor able for preparing cells for treating to the liver cirrhosis. So, we improved pretreat ment method for temperature sensitive culture dish and increased producing cell sheets efficiency.
When the ADMPC sheets were transplanted into a liver cirrhosis model rats, compared with the control group, the fibrosis rate was significantly improved in the sheet administration group.

Free Research Field

再生医療