Investigation of calyculin biosynthesis pathway

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H05996
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Natural medicines
• Research Institution: Hokkaido University
• Principal Investigator: EGAMI Yoko 北海道大学, 薬学研究院, 助教 (50758612)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: カリクリンA / 生合成

Outline of Final Research Achievements

In this study, we tried to investigation of calyculin A biosynthetic pathway. Uncultured symbiont bacteria Candidatus Entotheonella sp. had been identified as calyculin producer. To obtain functional evidences of uncultured bacterial genome, there is no ways other than manipulate gene in cultivable heterologous host, or establish the cultivation condition. To analysis of the biosynthetic mechanisms for nitrile formation, phosphorylation at C17, and chain-shortening process during calyculin biosynthesis, putative tailoring enzymes and substrates were prepared based on bioinfomation of calyculin biosynthetic gene, and subjected to in vitro analyses. As a result, new compound was detected in CalD enzyme reaction.

Free Research Field

天然物化学