2016 Fiscal Year Annual Research Report
Restoring tissue homeostasis by regulating TGF-beta signaling by using macrocyclic peptides
| Project/Area Number |
15H06073
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | macrocyclic peptides / in vitro selection / receptors / signaling / TGFbeta / cancer |
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| Outline of Annual Research Achievements |
The first selections against TbetaR2 and ALK5 were completed. Unfortunately, the peptide sequences recovered were not found to be desirable binders. Analysis of the sequences suggests that the method of attachment of the protein to the beads, 6x histidine tags, were less than adequate. To circumvent this problem, we've decided to bind the entire TBR2-TGFbeta3-ALK5 complex to the beads and used that as a selection target. The advantage of this is that the 6x histidine tags of each of the receptors will be used to immobilize the complex, ie four times 6x histidine tags. The selection against the active complex is underway. Despite having yet to finish our selection against the active complex, we have established our lab for the chemical synthesis for macrocyclic peptides. We have successfully synthesized and purified macrocyclic peptides identified from selections not related to the project in this proposal. Upon identification of TbetaR2- and/or ALK5-binding macrocyclic peptides, the identified peptides can be rapidly synthesized and purified in our lab.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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