Analysis of activity-dependent dynamics of NMDA receptors in mice with age-dependent synaptic dysfunction

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06078
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gunma University
• Principal Investigator: Hanamura Kenji 群馬大学, 大学院医学系研究科, 助教 (40361365)
• Co-Investigator(Renkei-kenkyūsha): TAKATSURU YUSUKE 群馬大学, 大学院医学系研究科, 講師 (30446265)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 樹状突起スパイン / 2光子励起レーザー顕微鏡 / ドレブリン / NMDA受容体

Outline of Final Research Achievements

In vivo imaging by using two-photon laser microscopy under deep anesthesia revealed dendritic spine morphology of pyramidal neurons in the cerebral cortex of transgenic mice expressing GFP under the control of Thy1-promoter. Analysis of heterozygotes of knockout mice deficient in drebrin A-specific exon or all drebrin isoforms tended to show some changes in turnover rate of dendritic spines compared with wild-type mice. The amount of NMDA receptors in synaptosomes were detected by western blotting.

Free Research Field

神経生物学、神経薬理学、神経化学