Transcriptome analysis of tumor microenvironment

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06172
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: Taguchi Ayumi 東京大学, 医学部附属病院, 登録研究員 (60756782)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 腫瘍内微小環境 / 好中球 / 炎症 / T細胞 / 癌遺伝子

Outline of Final Research Achievements

Mutant KRAS was transduced into mouse peritoneal cancer model, and tumor formation as well as characteristics of tumor microenvironment was observed. In the KRAS-transduced model, tumor formation was accelerated along with the exaggerated inflammation and increased number of neutrophils. In order to assess the association of neutrophils with peritoneal cancer, neutrophils were depleted with anti-Ly6G antibody. Depletion of neutrophils promoted tumor formation and production of ascites. Depletion of neutrophils decreased CD8 T cells and increased CD4 T cells in ascites. Neutrophils in KRAS-transduced ascites enhanced naive CD8 T cell proliferation with higher level of T cell costimulatory molecule, OX40-L.

Free Research Field

婦人科腫瘍