Pathophysiological function of calcitonin gene-related peptide in ocular inflammatory diseases.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06244
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Shinshu University
• Principal Investigator: TORIYAMA Yuichi 信州大学, 医学部, 助教(特定雇用) (90757759)
• Research Collaborator: SHINDO Takayuki 信州大学, 学術研究院医学系, 教授 (50253406) ; MURATA Toshinori 信州大学, 学術研究院医学系, 教授 (50253406) ; IMAI Akira 信州大学, 大学院医学系研究科, 大学院生 ; HIRABAYASHI Kazutaka 信州大学, 大学院医学系研究科, 大学院生
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: カルシトニン遺伝子関連ペプチド / アドレノメデュリン / 網膜

Outline of Final Research Achievements

Because the LPS-induced uveitis model did not work well, we tried to explore other models. In Kimba mice, which overexpress human vascular endothelial growth factor in their retinas, the capillary dropout, vascular leakage, and vascular fragility characteristic of diabetic retinopathy were observed. Intravitreal or systemic administration of ADM to Kimba mice ameliorated both the capillary dropout and vascular leakage. Evaluation of the transendothelial electrical resistance and fluorescein isothiocyanate-dextran permeability of an endothelial cell monolayer using TR-iBRB retinal capillary endothelial cells revealed that vascular endothelial growth factor enhanced vascular permeability but that co-administration of ADM suppressed the effect, in part by enhancing tight junction formation between endothelial cells. AM could serve as a novel therapeutic agent for the treatment of diabetic retinopathy.

Free Research Field

眼科学