Development of thermo-responsive magnetic conjugates designed for isolation and purification of circulating tumor cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06260
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials engineering
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Fuchigami Teruaki 名古屋工業大学, 工学(系)研究科(研究院), 助教 (20756704)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 磁性ナノ粒子 / 磁気細胞分離 / 血中循環腫瘍細胞 / 温度応答性高分子 / がん早期診断

Outline of Final Research Achievements

Novel thermo-responsive magnetic conjugates composed of iron oxide particles, streptavidin and thermos-responsive polymer were developed to achieve high-efficiency enrichment and purification of circulating tumor cells (CTCs) with no stress. Controlling an amount of streptavidin on a surface of the conjugates, capture efficiency of CTCs increased from 25 to 60%. Observing the conjugates motion on lung cancer cells under microscope, the thermo-responsive conjugates captured and released cancer cells by changing temperature from body temperature (37°C) to lower temperatures. According to these results, the novel thermos-responsive conjugates has an advantageous for the high-efficiency enrichment and the purification.

Free Research Field

nanotechnology