A study of anti-tumor effect of the dendritic cells infected with HF10-packaged amplicon carrying tumor associated antigen

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06279
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Nagoya University
• Principal Investigator: Luo Chenhong 名古屋大学, 医学系研究科, 招へい教員 (40759627)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: Glypican 3 / dendritic cells / Lamp-1 / signal peptide

Outline of Final Research Achievements

In this study, our results indicated that 1. HF10-package amplicon, which expresses tumor associated antigen glypican3 (GPC3), induced the antigen specific immune response through infected dendritic cells and inhibited the tumor growth in mouse ovarian cancer model. 2. In mouse peritoneal tumor seeding model, through prophylactic vaccination, we found that the dosing i.p. had the best antitumor effect among i.p., i.v., and s.c. dosing routes. 3. The chimera of Lamp-1 signal peptide and GPC3 induced GPC3 specific immune response through dendritic cell presentation more strongly than GCP3.

Free Research Field

ウイルス